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  • hhu-plant-biochemistry/Wrobel-2023-CastorBeanEndospermProteome
  • ceplas/Wrobel-2023-CastorBeanEndospermProteome
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......@@ -9,3 +9,7 @@ Wrobel TJ, Brilhaus D, Stefanski A, Stühler K, Weber APM, Linka N. Mapping the
Copyright © 2023 Wrobel, Brilhaus, Stefanski, Stühler, Weber and Linka
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
## Data availability
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD040932.
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## FAME-analysis
![fpls-14-1182105-g002.jpg](dataset/fpls-14-1182105-g002.jpg)
**Figure 2**
Storage oil mobilization in castor bean endosperm. (A) Relative fresh weight of the endosperm in dry seeds (-1d), imbibed seeds (0d) and 1- to 7-day old dark-grown castor bean seedlings (1-7d) as percentage of fresh weight of the whole plant. Data represent arithmetic means ± SD of 3 biological replicates. (B) Relative ricinoleic acid content of the endosperm from seeds and seedlings. Samples were normalized to the average fresh weight of on endosperm and expressed as percentages of their initial quantities determined in dry seeds (-1d). Error bars show standard deviations of the means of at least three biological replicates.
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assays/2_2-FAME-analysis/dataset/fpls-14-1182105-g002.jpg

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![fpls-14-1182105-g003.jpg](dataset/fpls-14-1182105-g003.jpg)
**Figure 3**
Isolation of organelles from etiolated castor bean seedlings. Four fractions of the sucrose density step gradient after centrifugation were taken from the gradient for various analyses at the interface 30% - 44% (w/w) sucrose solution (F1), 44% - 48% (w/w) sucrose solution (F2), 48% - 49% (w/w) sucrose solution (F3), and 50% - 54% (w/w) sucrose solution (F4).
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assays/2_3-OrganellesFromEndosperm/dataset/fpls-14-1182105-g003.jpg

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![fpls-14-1182105-t001.jpg](dataset\fpls-14-1182105-t001.jpg)
**Table 1**
Distribution of marker enzyme activities between the fractions.
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assays/2_4-EnzymeActivityOrganellarMarkers/dataset/fpls-14-1182105-t001.jpg

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