assays/2_6-SDS-PAGE-Immunoblotting/README.md

+![fpls-14-1182105-g004.jpg](dataset\fpls-14-1182105-g004.jpg)
+
+**Figure 4** 
+
+Purification of intracellular membranes from castor bean endosperm. Distribution of organellar membrane proteins within the organellar fractions (F1-F4) obtained after sucrose density gradient centrifugation. (A) SDS-PAGE analysis. Each lane was loaded with enriched membrane proteins from each pellet fraction after organelle disruption and ultracentrifugation (F1/F2: 25 µg, F3/F4: 15 µg). Equal loading of the protein gel within the fractions was monitored by staining with colloidal Coomassie G-250 dye. (B) Immunoblot analysis using antibodies raised against the following organelle membrane proteins: chloroplast TIC40, mitochondrial AOX, ER BIP2, and peroxisomal PEX14 proteins. Expected molecular weight for the corresponding castor bean proteins: 50.1 kDa for RcTIC40 (A3QSJ7), 39-41 kDa for RcAOX1/2 (B9RXE2/B9SO38), 73.3 kDa for RcBIP2 (B9RYP6), and 58.3 kDa for RcPEX14 (B9RB25). The membrane was serially probed with antibodies to the indicated marker proteins. The positions of molecular mass markers (in kDa) are indicated at the left.
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assays/2_9-StatisticalAnalysis-ProteomeDeconvolution/README.md

+![fpls-14-1182105-g005.jpg](dataset\fpls-14-1182105-g005.jpg)
 
-Source: https://www.frontiersin.org/articles/10.3389/fpls.2023.1182105/full#supplementary-material
+**Figure 5** 
+
+Principal Component Analysis of the Ricinus proteome of the fractions obtained from the sucrose density centrifugation, representing the total proteins (T1 to T4) and the enriched membrane proteins (M1 to M4). In this PCA plot each point represents the identified proteome of one biological experiment.
+
+![fpls-14-1182105-g006.jpg](dataset\fpls-14-1182105-g006.jpg)
+
+**Figure 6** 
+
+Distribution profile of compartment-specific marker proteins in the density-gradient fractions containing the total proteins (T1 to T4) and membrane proteins (M1 to M4). (A) peroxisomes; (B) plastid; (C) mitochondria; (D) ER; (E) nucleus; (F) Golgi; (G) vacuole; (H) cytosol. Relative LFQ represents the sum of Label Free Quantification (LFQ) values for all proteins belonging to an organelle relative to its maximal value.
+
+## Supplementary material
+
+Source for supplementary material: https://www.frontiersin.org/articles/10.3389/fpls.2023.1182105/full#supplementary-material
 
 ## "Table 3.xlsx"
 
@@ -11,7 +24,6 @@ Deconvolution-based assignment of the MS-identified proteins from etiolated cast
 SUPPLEMENTARY TABLE 4
 List of proteins found in peroxisomes, mitochondria, and plastids isolated from castor bean etiolated endosperm in this study. For functional annotation of proteins and their assignment to biological processes or metabolic pathways, gene ontology analysis was performed by Uniprot.
 
-
 ## "Image 1.jpg"
 
 SUPPLEMENTARY FIGURE 1