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CEPLAS RNASeq Workshop 2022
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HHU Plant Biochemistry
CEPLAS RNASeq Workshop 2022
Commits
fb2b9757
Commit
fb2b9757
authored
2 years ago
by
Dominik Brilhaus
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update sleuth (replace edgeR)
parent
878e083e
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_reader/RNAseqWorkshop.pdf
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_reader/RNAseqWorkshop.pdf
workflows/sleuth_differential_expression.R
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workflows/sleuth_differential_expression.R
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workflows/sleuth_differential_expression.R
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@@ -4,10 +4,6 @@ library(sleuth)
library
(
ggplot2
)
# First we need to specify where the kallisto results are stored.
# If you didn't specify this in your kallisto script, move all kallisto results
# folders (one for each sample) by GUI or the command line into a new folder called
# "kallisto_results".
# Begin by storing the base directory of the kallisto results in a variable
base_dir
<-
"runs/kallisto_results/"
...
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@@ -77,8 +73,8 @@ table(treatment.vs.mock$qval <= 0.01)
head
(
treatment.vs.mock
)
# <<< challenge excercises >>> #
# 1. compare the logFC
edgeR
calculated to that which we did
# 2. where does the difference comes from?
(it's in the edgeR manual)
# 1. compare the logFC
sleuth
calculated to that which we did
# 2. where does the difference comes from?
# now we transfer the result to our compilation data.frame 'dfr'
# actually, all we really want is the 'false discovery rate' AKA 'q_value'
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