# Identification of nuclear pore proteins at plasmodesmata
## Abstract
Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
Raw and meta data can be found in "assays":
NUP localization when transiently and stably expressed, topolgy of CPR5, structured illumination microscopy transiently expressing CPR5 and PDLP5, transport assays: bombardment, DropANdSee, short root SHR-GFP.
- Affiliation: CEBAS-CSIC, Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Insitute of Molecular Physiology Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Institute Molecular Physiology;Abiopep SL, ;Centro de Edafología y Biología Aplicada del Segura, Plant Virology;Centro de Edafología y Biología Aplicada del Segura, Plant Virology
- Affiliation: Université de Lorraine, UMR 1136 Université de Lorraine/INRAE Interactions Arbres Microorganismes (IAM);Heinrich-Heine-Universität Düsseldorf, Institute for Molecular Physiology;University of Liège, Integrative Biological Sciences (InBioS);Université catholique de Louvain, Louvain Institute of Biomolecular Science and Technology (LIBST);CNRS Délégation Ile-de-France Sud, Institut des Sciences du Végétal (ISV)
- Affiliation: Nagoya University, Institute of Transformative Bio-Molecules;Nagoya University, Institute of Transformative Bio-Molecules;Carnegie Institution for Science, Department of Plant Biology;Nara Institute of Science and Technology, Department of Biological Science
- Affiliation: University of Montpellier, IRD;Heinrich Heine University Düsseldorf, Institute for Molecular Physiology;University of Warwick, School of Life Sciences;CEA Grenoble, Cell & Plant Physiology Laboratory