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Commit 17c3db63 authored by Dominik Brilhaus's avatar Dominik Brilhaus
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polish README

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# Identification of nuclear pore proteins at plasmodesmata
## Abstract
Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
Raw and meta data can be found in "assays":
NUP localization when transiently and stably expressed, topolgy of CPR5, structured illumination microscopy transiently expressing CPR5 and PDLP5, transport assays: bombardment, DropANdSee, short root SHR-GFP.
Sequencing results for cpr5G420D can be found in
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## Raw data and metadata can be found in "assays"
- NUP localization
- [transiently expressed](assays/transient_localization)
- [stably expressed](assays/stable_localization_in_reporter_line)
- [topology of CPR5](assays/topology)
- [structured illumination microscopy (SIM)](assays/SIM) transiently expressing CPR5 and PDLP5
- transport assays
- [bombardment](assays/bombardment)
- [DropANdSee (DANS)](assays/DANS)
- [short root SHR-GFP](assays/SHR-GFP)
- [sequencing results for cpr5G420D](assays/sequencing_of_Arabidopsis_cpr5-1)
## Contributors
- Till Moritz Schladt
- email: till.schladt@hhu.de
- Affiliation: Heinrich Heine Univeryity, Düsseldorf
- ORCID: [0000-0002-9160-4664](https://orcid.org/0000-0002-9160-4664)
- Miras Manuel
- Affiliation: CEBAS-CSIC, Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Insitute of Molecular Physiology Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Institute Molecular Physiology;Abiopep SL, ;Centro de Edafología y Biología Aplicada del Segura, Plant Virology;Centro de Edafología y Biología Aplicada del Segura, Plant Virology
- ORCID: [0000-0002-3636-0265](https://orcid.org/0000-0002-3636-0265)
- Jona Obinna Ejike
- Affiliation: Heinrich Heine Universität, Düsseldorf
- ORCID: [0000-0002-4601-8551](https://orcid.org/0000-0002-4601-8551)
- Mathieu Pottier
- Affiliation: Université de Lorraine, UMR 1136 Université de Lorraine/INRAE Interactions Arbres Microorganismes (IAM);Heinrich-Heine-Universität Düsseldorf, Institute for Molecular Physiology;University of Liège, Integrative Biological Sciences (InBioS);Université catholique de Louvain, Louvain Institute of Biomolecular Science and Technology (LIBST);CNRS Délégation Ile-de-France Sud, Institut des Sciences du Végétal (ISV)
- ORCID: [0000-0003-1551-4699](https://orcid.org/0000-0003-1551-4699)
- Lin Xi
- Affiliation: University of Hohenheim
- ORCID: [0000-0002-6811-9505](https://orcid.org/0000-0002-6811-9505)
- Andrea Restrepo
- Affiliation: Heinrich-Heine-Universität Düsseldorf, Institute for Molecular Physiology
- ORCID: [0000-0003-2870-394X](https://orcid.org/0000-0003-2870-394X)
- Masayoshi Nakamura
- Affiliation: Nagoya University, Institute of Transformative Bio-Molecules;Nagoya University, Institute of Transformative Bio-Molecules;Carnegie Institution for Science, Department of Plant Biology;Nara Institute of Science and Technology, Department of Biological Science
- ORCID: [0000-0002-7107-4942](https://orcid.org/0000-0002-7107-4942)
- Niklas Pütz
- Affiliation: Heinrich Heine Universität, Düsseldorf
- ORCID: [0009-0006-1153-5918](https://orcid.org/0009-0006-1153-5918)
- Sebastian Hänsch
- Affiliation: Heinrich Heine University Düsseldorf, Center for Advanced Imaging;Heinrich Heine University Düsseldorf, Center for Advanced Imaging
- ORCID: [0000-0002-7762-2516](https://orcid.org/0000-0002-7762-2516)
- Chen Gao
- Affiliation: University of Copenhagen, Department of Plant and Environmental Sciences
- ORCID: [0000-0003-4750-3900](https://orcid.org/0000-0003-4750-3900)
- Julia Engelhorn
- Affiliation: University of Montpellier, IRD;Heinrich Heine University Düsseldorf, Institute for Molecular Physiology;University of Warwick, School of Life Sciences;CEA Grenoble, Cell & Plant Physiology Laboratory
- ORCID: [0000-0002-6680-3012](https://orcid.org/0000-0002-6680-3012)
- Marcel Dickmanns
- Affiliation: Heinrich-Heine-Universität Düsseldorf, ;Max Planck Institute of Biochemistry,
- ORCID: [0000-0003-4001-707X](https://orcid.org/0000-0003-4001-707X)
- Gwendolyn Davis
- Affiliation: University of Warwick, Life Sciences;University of Cambridge, Plant Sciences
- ORCID: [0000-0003-4704-4863](https://orcid.org/0000-0003-4704-4863)
- Ahan Dalal
- Affiliation: Hebrew University of Jerusalem, Plant Science;Tel Aviv University, Plant Science
- ORCID: [0000-0003-1876-1484](https://orcid.org/0000-0003-1876-1484)
- Sven Gombos
- Affiliation: University of Hohenheim, Plant Systems Biology
- ORCID: [0000-0003-4177-526X](https://orcid.org/0000-0003-4177-526X)
- Ronja Lange
- Affiliation: Heinrich Heine Universität, Düsseldorf
- ORCID: [0009-0009-2475-2438](https://orcid.org/0009-0009-2475-2438)
- Rüdiger Simon
- Affiliation: Heinrich Heine University, Biology
- ORCID: [0000-0002-1317-7716](https://orcid.org/0000-0002-1317-7716)
- Waltraud Schulze
- Affiliation: University of Hohenheim
- ORCID: [0000-0001-9957-7245](https://orcid.org/0000-0001-9957-7245)
- Wolf B. Frommer
- Affiliation: Heinrich-Heine-Universitat Düsseldorf, Institute for Molecular Physiology;ITbM, ;Carnegie Institution for Science, Plant Biology;Stanford University, Biology;Carnegie Institution for Science, Plant Biology;Stanford University, Biology;Carnegie Institution for Science, Plant Biology;University of Tübingen, Biology
- ORCID: [0000-0001-6465-0115](https://orcid.org/0000-0001-6465-0115)
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