Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
## Preprint
A bioRxiv preprint of the associated publication is available at https://doi.org/10.1101/2024.09.02.610746 and [here](_publication/biorxiv-preprint/2024.09.02.610746v2.full.pdf)
See [2024.09.02.610746v2.full.pdf](biorxiv-preprint/2024.09.02.610746v2.full.pdf)
### Citation
**Identification of nuclear pore proteins at plasmodesmata**
T. Moritz Schladt, Manuel Miras, Jona Obinna Ejike, Mathieu Pottier, Lin Xi, Andrea Restrepo-Escobar, Masayoshi Nakamura, Niklas Pütz, Sebastian Hänsch, Chen Gao, Julia Engelhorn, Marcel Dickmanns, Gwendolyn V. Davis, Ahan Dalal, Sven Gombos, Ronja Lange, Rüdiger Simon, Waltraud X. Schulze, Wolf B. Frommer