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# Identification of nuclear pore proteins at plasmodesmata
 
 
## Abstract
 
Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
Here we used bioinformatics, proteomics and fluorescence imaging to identify proteins with similarities to phase separating FG-NUPs at PD. We identified 20 NUPs in PD fractions, and validated dual localization to NPC and PD for 7 NUPs. Structured illumination microscopy detected the transmembrane anchor NUP CPR5 at both orifices of PD. cpr5 mutants showed reduced intercellular transport of SHR from stele to endodermis. The identification of FG-NUPs at PD is consistent with the recruitment of NUPs in the green lineage to form a PD pore gating complex consistent with liquid phase separation domains as diffusion barriers at PD.
Raw and meta data can be found in "assays":
NUP localization when transiently and stably expressed, topolgy of CPR5, structured illumination microscopy transiently expressing CPR5 and PDLP5, transport assays: bombardment, DropANdSee, short root SHR-GFP.
Sequencing results for cpr5G420D can be found in
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## Raw data and metadata can be found in "assays"
 
 
- NUP localization
 
- [transiently expressed](assays/transient_localization)
 
- [stably expressed](assays/stable_localization_in_reporter_line)
 
- [topology of CPR5](assays/topology)
 
- [structured illumination microscopy (SIM)](assays/SIM) transiently expressing CPR5 and PDLP5
 
- transport assays
 
- [bombardment](assays/bombardment)
 
- [DropANdSee (DANS)](assays/DANS)
 
- [short root SHR-GFP](assays/SHR-GFP)
 
- [sequencing results for cpr5G420D](assays/sequencing_of_Arabidopsis_cpr5-1)
 
 
## Contributors
 
 
- Till Moritz Schladt
 
- email: till.schladt@hhu.de
 
- Affiliation: Heinrich Heine Univeryity, Düsseldorf
 
- ORCID: [0000-0002-9160-4664](https://orcid.org/0000-0002-9160-4664)
 
- Miras Manuel
 
- Affiliation: CEBAS-CSIC, Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Insitute of Molecular Physiology Heinrich-Heine-Universität Düsseldorf Mathematisch-Naturwissenschaftliche Fakultät, Institute Molecular Physiology;Abiopep SL, ;Centro de Edafología y Biología Aplicada del Segura, Plant Virology;Centro de Edafología y Biología Aplicada del Segura, Plant Virology
 
- ORCID: [0000-0002-3636-0265](https://orcid.org/0000-0002-3636-0265)
 
- Jona Obinna Ejike
 
- Affiliation: Heinrich Heine Universität, Düsseldorf
 
- ORCID: [0000-0002-4601-8551](https://orcid.org/0000-0002-4601-8551)
 
- Mathieu Pottier
 
- Affiliation: Université de Lorraine, UMR 1136 Université de Lorraine/INRAE Interactions Arbres Microorganismes (IAM);Heinrich-Heine-Universität Düsseldorf, Institute for Molecular Physiology;University of Liège, Integrative Biological Sciences (InBioS);Université catholique de Louvain, Louvain Institute of Biomolecular Science and Technology (LIBST);CNRS Délégation Ile-de-France Sud, Institut des Sciences du Végétal (ISV)
 
- ORCID: [0000-0003-1551-4699](https://orcid.org/0000-0003-1551-4699)
 
- Lin Xi
 
- Affiliation: University of Hohenheim
 
- ORCID: [0000-0002-6811-9505](https://orcid.org/0000-0002-6811-9505)
 
- Andrea Restrepo
 
- Affiliation: Heinrich-Heine-Universität Düsseldorf, Institute for Molecular Physiology
 
- ORCID: [0000-0003-2870-394X](https://orcid.org/0000-0003-2870-394X)
 
- Masayoshi Nakamura
 
- Affiliation: Nagoya University, Institute of Transformative Bio-Molecules;Nagoya University, Institute of Transformative Bio-Molecules;Carnegie Institution for Science, Department of Plant Biology;Nara Institute of Science and Technology, Department of Biological Science
 
- ORCID: [0000-0002-7107-4942](https://orcid.org/0000-0002-7107-4942)
 
- Niklas Pütz
 
- Affiliation: Heinrich Heine Universität, Düsseldorf
 
- ORCID: [0009-0006-1153-5918](https://orcid.org/0009-0006-1153-5918)
 
- Sebastian Hänsch
 
- Affiliation: Heinrich Heine University Düsseldorf, Center for Advanced Imaging;Heinrich Heine University Düsseldorf, Center for Advanced Imaging
 
- ORCID: [0000-0002-7762-2516](https://orcid.org/0000-0002-7762-2516)
 
- Chen Gao
 
- Affiliation: University of Copenhagen, Department of Plant and Environmental Sciences
 
- ORCID: [0000-0003-4750-3900](https://orcid.org/0000-0003-4750-3900)
 
- Julia Engelhorn
 
- Affiliation: University of Montpellier, IRD;Heinrich Heine University Düsseldorf, Institute for Molecular Physiology;University of Warwick, School of Life Sciences;CEA Grenoble, Cell & Plant Physiology Laboratory
 
- ORCID: [0000-0002-6680-3012](https://orcid.org/0000-0002-6680-3012)
 
- Marcel Dickmanns
 
- Affiliation: Heinrich-Heine-Universität Düsseldorf, ;Max Planck Institute of Biochemistry,
 
- ORCID: [0000-0003-4001-707X](https://orcid.org/0000-0003-4001-707X)
 
- Gwendolyn Davis
 
- Affiliation: University of Warwick, Life Sciences;University of Cambridge, Plant Sciences
 
- ORCID: [0000-0003-4704-4863](https://orcid.org/0000-0003-4704-4863)
 
- Ahan Dalal
 
- Affiliation: Hebrew University of Jerusalem, Plant Science;Tel Aviv University, Plant Science
 
- ORCID: [0000-0003-1876-1484](https://orcid.org/0000-0003-1876-1484)
 
- Sven Gombos
 
- Affiliation: University of Hohenheim, Plant Systems Biology
 
- ORCID: [0000-0003-4177-526X](https://orcid.org/0000-0003-4177-526X)
 
- Ronja Lange
 
- Affiliation: Heinrich Heine Universität, Düsseldorf
 
- ORCID: [0009-0009-2475-2438](https://orcid.org/0009-0009-2475-2438)
 
- Rüdiger Simon
 
- Affiliation: Heinrich Heine University, Biology
 
- ORCID: [0000-0002-1317-7716](https://orcid.org/0000-0002-1317-7716)
 
- Waltraud Schulze
 
- Affiliation: University of Hohenheim
 
- ORCID: [0000-0001-9957-7245](https://orcid.org/0000-0001-9957-7245)
 
- Wolf B. Frommer
 
- Affiliation: Heinrich-Heine-Universitat Düsseldorf, Institute for Molecular Physiology;ITbM, ;Carnegie Institution for Science, Plant Biology;Stanford University, Biology;Carnegie Institution for Science, Plant Biology;Stanford University, Biology;Carnegie Institution for Science, Plant Biology;University of Tübingen, Biology
 
- ORCID: [0000-0001-6465-0115](https://orcid.org/0000-0001-6465-0115)
 
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