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 # Carbon Availability Transcriptomics
 
-Chlamydomonas reinhardtii CC-1690 was grown in bioreactors. When the cells reached a non-stationary density, the acetate supply was stopped and the medium was kept at 25°C (control) or heated to 35 °C and 40 °C. Additionally to a preheat sample, four further samples were taken as triplicates after 2h, 4h, 8h, and 24h of heat treatment (nopump samples). The samples were analysed with NGS. In [Zhang et al 2022](https://doi.org/10.1038/s42003-022-03359-z  ) samples were taken at the same time points for 35°C and 40°C but with constant nutrient supply (TAP samples). 
+<i>Chlamydomonas reinhardtii</i> CC-1690 was grown in bioreactors. When the cells reached a non-stationary density (2E+6 cells / ml), the acetate supply was stopped and the medium was kept at 25°C (control) or heated to 35 °C and 40 °C. Additionally to a preheat sample, four further samples were taken as triplicates after 2h, 4h, 8h, and 24h of heat treatment (nopump samples). The samples were analysed with NGS. In [Zhang et al 2022](https://doi.org/10.1038/s42003-022-03359-z  ) samples were taken at the same time points for 35°C and 40°C but with constant nutrient supply (TAP samples). 
 
 
 _Table 1: Sampling schema for Transcriptomics analysis. Three biological replicates were measured. An x indicates triplicates measured at the respective time points._
 
-|condition|-18 h (preheat)|2 h|4 h|8 h|24 h|reference|
-|---|--|--|--|--|--|--|
-|25°C TAP|x|x| | |x|[Zhang et al. 2023](https://doi.org/10.1101/2022.12.04.519034)|  
-|35°C TAP|x|x|x|x|x|[Zhang et al. 2022](https://doi.org/10.1038/s42003-022-03359-z)|  
-|40°C TAP|x|x|x|x|x|[Zhang et al. 2022](https://doi.org/10.1038/s42003-022-03359-z)|  
-|25°C nopump|x|x|x|x|x|[Zhang et al. 2023](https://doi.org/10.1101/2022.12.04.519034)|  
-|35°C nopump|x|x|x|x|x|[Zhang et al. 2023](https://doi.org/10.1101/2022.12.04.519034)|  
-|40°C nopump|x|x|x|x|x|[Zhang et al. 2023](https://doi.org/10.1101/2022.12.04.519034)|  
+|condition|-18 h<br>(preheat)|2 h|4 h|8 h|24 h|reference|notes|
+|---|--|--|--|--|--|--|--|
+|25°C, constant-acetate|x|x| | |x|[Zhang et al. 2023](https://doi.org/10.1093/jxb/erad405)|additional time points at<br>0h, 0.5h, 1h, 26h, and 48h |
+|35°C, constant-acetate|x|x|x|x|x|[Zhang et al. 2022](https://doi.org/10.1038/s42003-022-03359-z)|--|
+|40°C, constant-acetate|x|x|x|x|x|[Zhang et al. 2022](https://doi.org/10.1038/s42003-022-03359-z)|--|
+|25°C, acetate-depleting|x|x|x|x|x|[Zhang et al. 2023](https://doi.org/10.1093/jxb/erad405)|--|
+|35°C, acetate-depleting|x|x|x|x|x|[Zhang et al. 2023](https://doi.org/10.1093/jxb/erad405)|--|
+|40°C, acetate-depleting|x|x|x|x|x|[Zhang et al. 2023](https://doi.org/10.1093/jxb/erad405)|--|
 
 Comparative analysis of both experiments were performed within this ARC. These include