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README.md 838 B

Leaf relative water content

  • An approximately 1 cm 3 section of leaf was sampled from each plot and transferred to a pre-weighed Eppendorf tube.

  • The tubes were then reweighed, filled with MilliQ water and left at 4 °C overnight.

  • The following morning, the turgid leaf section was removed from the tube, carefully dried with a paper towel and reweighed.

  • Samples were then placed back into the emptied Eppendorf tubes and placed in an oven at approximately 70 °C overnight.

  • Once leaf sections were completely dry, the tubes containing the leaf samples were reweighed.

  • The empty tube weight was subtracted from the fresh weight and dried weight measurements and relative water content (RWC, %) was calculated using the equation:

RWC = \frac{fresh~weight - dry~weight}{turgid~weight - dry~weight} ~x ~100