README.md

Leaf relative water content

• An approximately 1 cm 3 section of leaf was sampled from each plot and transferred to a pre-weighed Eppendorf tube.

• The tubes were then reweighed, filled with MilliQ water and left at 4 °C overnight.

• The following morning, the turgid leaf section was removed from the tube, carefully dried with a paper towel and reweighed.

• Samples were then placed back into the emptied Eppendorf tubes and placed in an oven at approximately 70 °C overnight.

• Once leaf sections were completely dry, the tubes containing the leaf samples were reweighed.

• The empty tube weight was subtracted from the fresh weight and dried weight measurements and relative water content (RWC, %) was calculated using the equation:

$$RWC = \frac{fresh~weight - dry~weight}{turgid~weight - dry~weight} ~x ~100$$