Add new protocol file

assays/RelativeWaterContent/protocols/Protocol_RWC

+Leaf relative water content
+
+
+An approximately 1 cm 3 section of leaf was sampled from each plot
+and transferred to a pre-weighed Eppendorf tube.
+
+
+The tubes were then reweighed, filled with MilliQ water
+and left at 4 °C overnight.
+
+
+The following morning, the turgid leaf section was removed from the tube, carefully dried with a paper towel and reweighed.
+
+
+Samples were then placed back into the emptied
+Eppendorf tubes and placed in an oven at approximately 70 °C overnight.
+
+
+Once leaf sections were completely
+dry, the tubes containing the leaf samples were reweighed.
+
+
+The empty tube weight was subtracted from the
+fresh weight and dried weight measurements and relative water content (RWC, %) was calculated using the
+equation:
+
+
+RWC=fresh weight−dry weightturgid weight−dry weight x 100RWC = \frac{fresh~weight - dry~weight}{turgid~weight - dry~weight} ~x ~100 RWC=turgid weight−dry weightfresh weight−dry weight​ x 100
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