Fluorescence data was collected using a MINI-PAM-II fluorometer (Walz Heinz GmbH, Effeltrich,
Fluorescence data was collected using a MINI-PAM-II fluorometer (Walz Heinz GmbH, Effeltrich,
Germany). To conduct measurements, the leaf clip was attached to a leaf and measurements of
Germany).
**To conduct measurements:**
- The leaf clip was attached to a leaf and measurements of
photosynthetically active radiation (PAR) and the photosynthetic efficiency of photosystem II (PhiPSII) were
photosynthetically active radiation (PAR) and the photosynthetic efficiency of photosystem II (PhiPSII) were
recorded. This was repeated ~10 times per plot. Each FI plot was followed by its RI plot counterpart. Plots of
recorded.
blocks 1 and 4 were measured 49 days after sowing between 14:45 and 15:45. Plots of blocks 2 and 5 were
- This was repeated ~10 times per plot.
- Each FI plot was followed by its RI plot counterpart.
>**Plots of blocks 1 and 4 were measured 49 days after sowing between 14:45 and 15:45. Plots of blocks 2 and 5 were
measured 48 days after sowing between 15:00 and 17:00. All blocks were measured 50 days after sowing
measured 48 days after sowing between 15:00 and 17:00. All blocks were measured 50 days after sowing
between 10:24 and 12:49. The fluorescence kinetics of saturation pulse analyses for measurements wereinspected using WinControl-3 Software (version 3.29-rev.1112) (Walz Heinz GmbH, Effeltrich, Germany).
between 10:24 and 12:49.**
The fluorescence kinetics of saturation pulse analyses for measurements were inspected using WinControl-3 Software (version 3.29-rev.1112) (Walz Heinz GmbH, Effeltrich, Germany).
Measurements that did not pass inspection were discarded from subsequent analyses.
Measurements that did not pass inspection were discarded from subsequent analyses.
PhiPSII values were clustered according to genotype, treatment and PAR level using custom R scripts.
PhiPSII values were clustered according to genotype, treatment and PAR level using custom R scripts.
PAR was divided into four levels: low (<500 μmol photons m -2 s -1 ), mid-low (500-1000 μmol photons m -2 s -1 ),
mid-high (1000-1500 μmol photons m -2 s -1 ) and high (>1500 μmol photons m -2 s -1 ). Agglomerative hierarchical
**PAR was divided into four levels:**
clustering was applied on the scaled PhiPSII means for all plots using the ‘agnes’ function of the R package
- low (<500 μmol photons m -2 s -1 ),\
‘cluster’ [100] with a cut-off of five clusters.
- mid-low (500-1000 μmol photons m -2 s -1 ),
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- mid-high (1000-1500 μmol photons m -2 s -1 ) and
- high (>1500 μmol photons m -2 s -1 ).
Agglomerative hierarchical clustering was applied on the scaled PhiPSII means for all plots using the ‘agnes’ function of the R package ‘cluster’ [1] with a cut-off of five clusters.
Reference:
[1] Maechler, M.; Rousseeuw, P.; Struyf, A.; Hubert, M.; Hornik, K.; Studer, M.; Roudier, P.; Gonzalez, J.; Kozlowski, K.; Schubert, E.; et al. “Finding Groups in Data”: Cluster Analysis Extended Rousseeuw et al. Available online: https://cran.r-project.org/web/packages/cluster/cluster.pdf (accessed on 26 February 2021).