## Physiology, biochemistry and anatomy of young fully geown leaves from Brassicaceae species with C<sub>3 </sub>and C<sub>3</sub>-C<sub>4 </sub>(C<sub>2</sub>) photosynthesis ##
**Aim of study**:<br> Identification of physiological, biochemical and anatomical features related to the C<sub>3</sub>-C<sub>4</sub> photosynthesis phenotype in the Brassicaceae <br>
**Species/taxa**: C<sub>3</sub>-C<sub>4</sub> phenotype in bold, *C. gynandra* is C<sub>4</sub> phenotype, all others with C<sub>3</sub> phenotype<br>
-*Arabidopsis thaliana* (L.) Heynh. (At),
-***Brassica gravinae*** Ten. (4 accessions, Bg1, Bg2, Bg3 and Bg4),
-*Brassica juncea* (L.) Czern. (Bj),
-*Brassica napus* L. (Bn),
-*Brassica nigra* (L.) W.D.J. Koch subsp.nigra va nigra (Bni),
-*Brassica oleraceae* L. (Bo),
-*Brassica rapa* L. (Br),
-*Brassica repanda* (Willd.) (Be),
-*Brassica tournefortii* Gouan. (2 accessions, Bt1 and Bt2),
-*Carrichtera annua* (L.) DC. (Ca),
-*Diplotaxis acris* Boiss. (Da),
-***Diplotaxis erucoides*** (L.) DC. (De),
-*Diplotaxis harra* Boiss. (Dh),
-***Diplotaxis muralis*** (L.) DC. (Dm),
-***Diplotaxis tenuifolia*** (L.) DC. (Dt),
-*Diplotaxis tenuisiliqua* Delile (Ds),
-*Diplotaxis viminea* (L.) DC. (Dv),
-*Eruca sativa* Mill. (Es),
-*Hirschfeldia incana* (L.) Lagr.-Foss (2 accessions HIR1 and **HIR3**),
-*****Gynandropsis gynandra***** (L.) Briq. /*****Cleome gynanadra***** L. <br>
**Analysis/measurements**:
- gas exchange by Li6800 IRGA measuring A-ci response and calculation of CO<sub>2 </sub> compensation point
- primary metabolite pattern by GCMS
- element analysis (CN ratio, <sup>13</sup>C) by EA-IRMS
- vein density (vein length per area)
- PEPC activity in leaf extracts by spectrophotometry
- Specific leaf area (area per dry weight)
- analysis of leaf cross sections by light microscopy and determination of organelle area in bundle sheath facing vein or mesopyll/intercellular space
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*Data presentation*: <br>
This ARC contains the datasets containing data measured per single plant and the raw data for the GCMS and EA-IRMS analysis.
**Publication**: <br> Results and discussion of the presented data has been published in the following paper: <br>**Schlüter, U., Bouvier, J. W., Guerreiro, R., Malisic, M., Kontny, C., Westhoff, P., Stich, B. & Weber, A.
P. M.** (2023), Brassicaceae display diverse photorespiratory carbon recapturing mechanisms, *Journal of Experimental Botany*, https://doi.org/10.1093/jxb/erad250<br>
Analysis of the genomes of the selected species/taxa can be found in the following paper:<br>**Guerreiro R, Bonthala VS, Schlüter U, Triesch S, Weber APM, Stich B.** 2023. A genomic panel for studying C3–C4 intermediate photosynthesis in the Brassiceae tribe. Plant, Cell & Environment https://doi.org/10.1111/pce.14662. <br>
Analysis of the GLDP1 promoter and the potential involvement of transposible elements in evolution of C<sub>3</sub>-C<sub>4</sub> phenotype in the Brassicaceae is discussed in:<br>**Triesch S, Denton AK, Buchmannn JP, Reichel-Deland V, Guerreiro R, Schlüter U, Weber APM.** 2023. Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species. doi: https://doi.org/10.1101/2022.12.06.519256
