added basic structure and filled in some metadata

assays/MALDI-TOF analysis of Si EPS and CW/protocols/MALDI-TOF analysis of Si EPS and CW.txt

+# Analysis of oligosaccharides released from the EPS matrix and CW of S. indica
+
+Freeze-dried  EPS  matrix  (1  mg)  or  CW  preparation  (1  mg)isolated fromS. indicawere suspended in 400mLofa2-mM sodium  acetate  buffer,  pH  5.0  (TLE),  2-mM  MES  buffer,  pH5.0  (H.  pomatiab-1,3-glucanase),  or  25-mM  sodium  acetatebuffer,  pH  5.0  (HvBGLUII)  and  incubated  at  70C  overnight.The  suspended  material  was  treated  with  2.5mLofTLE,2.5mLofH.  pomatiab-1,3-glucanase,  or  1mLofHvBGLUII  inthe respective buffers, as described before, in a total reactionvolume  of  50mL.  The  digestion was  performed  at  40Cwithshaking  at  500  rpm  for  16  h.  The  digestion  reaction  wasstopped at 95C for 10 min and centrifuged at 11,000gfor 5min at room temperature.
+
+# Mass spectrometrical analysis
+
+The  oligosaccharides  present  in  the  prepared  samples  wereanalyzed   by   Oligosaccharide   Mass   Profiling   as   described(Gu ̈nl et al., 2011).  Briefly,  the  samples  were  spotted  onto  adried  spot  of  dihydroxy  benzoic  acid  matrix  (10  mgmL–1)and   analyzed   by   MALDI-TOF   mass   spectrometry   (BrukerrapifleX  instrument).  The  machine  was  set  to  linear,  positivereflectron  mode  with  an  accelerating  voltage  of  20,000  V.The spectra from the samples were analyzed using flexanaly-sis software 4.0 (Bruker Daltonics).