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Commit 1514c8d7 authored by Viktoria Petrova's avatar Viktoria Petrova
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add camalexin analysis protocol

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## Camalexin
Camalexin was extracted from ~50 mg of leaves in 200 µl of dimethlysulfoxide (DMSO) as described in Koprivova et al. (2019). After centrifugation at room temperature for 20 min, 20 µl aliquots of the supernatant were injected into a Thermo Scientific Dionex UltiMate 3000 HPLC system with a Waters Spherisorb ODS-2 column (250 mm×4.6 mm, 5 µm). The samples were resolved using a gradient of acetonitrile in 0.01% (v/v) formic acid. Camalexin was detected by a fluorescence detector with an excitation at 318 nm and emission at 368 nm. For the quantification, external standards were used ranging from 1 pg µl–1 to 1 ng µl–1 (Koprivova et al., 2019).
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