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Commit 28cff6bf authored by Viktoria Petrova's avatar Viktoria Petrova
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add climate chamber experiment study and protocol

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## Climate chamber experimental conditions and design
Based on the results of the field experiments, six representative barley inbreds (HOR1842, IG128216, IG31424, ItuNative, K10877, and W23829/803911) were selected for a climate chamber experiment. The experimental design was a randomized complete block design with three replicates/blocks. A total of 32 plants were planted for each inbred in total. The gas exchange measurements were performed 21, 24, 26, 31, 36, 39, 46, 51, 57, 74, 87, 88, 100, and 102 days after sowing (DAS). At each measurement day, three measurements were taken from three different plants of an inbred, where always one plant from each of the blocks was studied. In addition, the Zadoks score for each evaluated plant was rated. In addition, we harvested three plants per inbred (i.e. one per block) and then measured dry weight of aboveground biomass per plant 26, 36, 46, 57, 74, 102, 113, and 142 DAS. The growth conditions in the climate chamber were as follows: 14 h/10 h light/dark photoperiod, 18 °C/16 °C temperature, and 55% relative humidity. The maximal light intensity measured at 15 cm from the light panel was 750 μmol m−2 s−1.
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