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Commit b992ea80 authored by Viktoria Petrova's avatar Viktoria Petrova
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UPDATE ISA assays and studies

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## Absorption spectra and OD measurements
Absorption spectra and OD measurements were carried out in 1 ml polystyrene cuvettes in a SPECORD 200 Plus Spectrophotometer (Analytik Jena) with BG11 as a blank and as a reference sample. Samples were diluted with BG11 to be within an absorption range of 0.1 to 1.0 to ensure accurate measurements. Cell densities for *Synechocystis* were measured at 750 nm.
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## Quantification of squalene production
For measurements of squalene production over time, 30 ml of BG11 were inoculated from a pre-culture to OD750 = 0.4, supplemented with 5 mM of rhamnose and incubated over 14 days. Samples were taken daily for the first four days, every second day for the following six days and after 14 days. The lost culture volume from sampling was replaced with fresh BG11 containing appropriate antibiotics and 5 mM of rhamnose.
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