metabolomics update and DAPGdetection assay

assays/DetectionOfR401DAPGandPyoverdine/protocols/DAPGandPyoverdineDetection.md

+## Detection of R401 DAPG and pyoverdine
+
+For the detection of iron-chelating compounds, a R401 preculture was grown over night in 5 ml LB medium at 30 °C and 200 rpm. Before inoculation of the main culture, cells were washed twice with the main culture medium to remove potential traces of iron from the medium. Erlenmeyer flasks containing 20 ml of modified MM63 (KH2PO4 13.61 g/L, KOH 4.21 g/L, (NH4)SO4 1.98 g/L, MgSO4*7 H2O 0.25 g/L, NaCl 0.5g/L, Glucose * H2O 5,00 g/L, pH 7.1 KOH/HCl) with or without addition of 0.0011 g/L FeSO4 * 7 H2O were inoculated with 100 μl of preculture and cultivated for 6 days at 30 °C and 200 rpm. Every second day, 0.5 ml sample were taken, cells were removed by centrifugation and 5 μl of  supernatant were analysed on a Bruker microTOFq-II high-resolution mass spectrometer coupled to an Agilent 1290 UPLC system with an Acquity UPLC BEH C-18 reverse phase column, run in a gradient of MeCN/H2O + 0.1% formic acid. Higher accuracy measurements were performed on a maXis-II qTOF, coupled to an identical LC setup as described earlier.
\ No newline at end of file