Skip to content
Snippets Groups Projects
Commit 2ad48042 authored by Viktoria Petrova's avatar Viktoria Petrova
Browse files

add comparison of autophagic activity assay and protocol

parent 9615c4ce
No related branches found
No related tags found
1 merge request!1Ceplas dm viktoria
File added
## Comparison of autophagic activity in roots mounted on glass or in RoPods
To assess the impact of glass-based sample mounting on the autophagic activity, RoPod v24 and standard agar plates were prepared similarly to the experiment for the root length measurement. After 6 days, seedlings growing on top of the agar medium were transferred in an empty glass bottom chamber, on a drop of 0.5 × MS and then gently covered with a cover slide. To prevent fast evaporation of the liquid medium, the glass bottom chamber was covered with a lid. Both RoPod v24 with seedlings and glass bottom chamber with transferred seedlings were imaged at different end points using CLSM 800 (Carl Zeiss), Objective 40x/1.2W, excitation light 488 nm, and emission 505–560 nm. The number of puncta was quantified in *Fiji* using the workflow presented in Supplementary File S2.
\ No newline at end of file
0% Loading or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment