add plant material study and protocol

studies/PlantMaterial/protocols/Plant-Material.md

+## Plant Material
+
+Autophagy deficient *atg5/atg7* double knockout line was established by crossing previously published *atg5-1* (Thomson et al., 2005) and *atg7-2* (Hofius, D. et al., 2009) mutants.
+
+Col-0 wild-type and *atg5/atg7* plants were transformed using GV3101 strain of Agrobacterium tumefaciens carrying pMDC pHusion1 AtATG8a construct as described in (Dauphinee, A. N. et al., 2019).
+
+Col-0 WT and *atg5-1* plants expressing GFP-ATG8a line was described previously (Minina, E. A. et al.,2018), (Thomson et al., 2005).
+
+Col-0 WT plants co-expressing vacuolar marker spL-RFP and the autophagosomal marker GFP-ATG8a were obtained by crossing the corresponding marker lines previously published in Hunter et al. ( Hunter et al., 2007) and Thomson et al. (Thomson et al., 2005).
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