Monitoring sucrose effect on root hair elongation illustrates applicability of RoPod for long-term time-lapse imaging combined with treatments. Arabidopsis Col-0 WT seedlings were grown for one week in a RoPod5 using the described protocol. The growth of root hairs was firstly recorded for 4 h under control conditions. After that, the chambers were flooded with liquid 0.5 × MS (control treatment) or liquid 0.5 × MS supplemented with 1% of sucrose (sucrose treatment). (a) Representative images showing roots at 0 h and 8 h of treatment. Each panel is a z-projection covering 240 µm. (b,c) Result of root hair tip tracking for one representative root for control (b) and for sucrose (c) treatments. The final root hair length (d), the root hair growth rate (e) and the growth duration (f) were calculated as described in the Supplementary Methods. (d–f) The sample size used for each measurement is shown in the tables below the charts. A Dunn’s multiple comparison test, different letters designate significantly different groups, p < 0.01. The dot in the violin plots is the median of the distribution.
