RoPod, a toolkit comprising plant growth protocol and microscopy chamber optimized for long-term time-lapse imaging of Arabidopsis roots.
(g–i) Growth conditions in RoPod chambers are equivalent to standard growing conditions on Petri plates, as demonstrated by comparing root growth on the top of the medium, under the medium inside the standard Petri plate and in RoPod chambers. Seeds of wild-type (WT) and autophagy-deficient atg5-1 Arabidopsis plants expressing GFP-ATG8 were sown on the agar medium in Petri plates or RoPods. Plates and chambers were placed on SPIRO under long day conditions and imaged for a week. (h) Zoomed-in insets shown in (g). (i) The chart represents data from two independent experiments. The root lengths were measured on the 7th day after seed plating. One-Way ANOVA test revealed no difference in root length of seedlings for the WT seedlings grown in RoPods and on Petri plates, significance level of 0.05, n = 103.
