add miRNA targeting prediction assay and protocol

assays/miRNATargetingPrediction/protocols/TargetingPredictionOfmiRNA.md

+## miRNA targeting prediction
+
+To predict potential regulation of *R*-genes by the miR482-superfamily (de Vries et al., 2015), we used psRNATarget (release 2017; Dai et al., 2018). We used the coding sequence (CDS) of our *R*-genes as the target library. To ensure a low rate of false-positives, the maximum expectation was set to ≤3, since higher expectation values represent less likely mRNA/miRNA interactions. We evaluated the likelihood of an *R*-gene being targeted by the miR482 superfamily and whether the *R*-gene encoded a full length NBS-LRR and or belonged to a *R*-gene cluster using a chi-square test (Greenwood and Nikulin, 1996).
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