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Viktoria Petrova authoredViktoria Petrova authored
Figure 3 C) caption
Figure 3 C). The activity of GBP1 and GBP1E500A was tested on laminarihexaose (β-1,3-glucan hexamer), laminaritriose (β-1,3-glucan trimer), cellohexaose (β-1,4-glucan hexamer), and XXXG (xyloglucan heptasaccharide). After overnight digestion at 25°C, the products were analyzed by MALDI-TOF mass spectrometry. The ion signal ladder between 500 and 1,000 Da represents an unknown contamination present in the GBP1 preparation and is thus unrelated to any digested carbohydrate. Digestion assays were performed twice with similar results. UT, untreated; XXXG, xyloglucan heptasaccharide.