add figure captions

assays/MALDI-TOFAnalysis/README.md

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 **Figure 3 C).** The activity of GBP1 and GBP1E500A was tested on laminarihexaose (β-1,3-glucan hexamer), laminaritriose (β-1,3-glucan trimer), cellohexaose (β-1,4-glucan hexamer), and XXXG (xyloglucan heptasaccharide). After overnight digestion at 25°C, the products were analyzed by MALDI-TOF mass spectrometry. The ion signal ladder between 500 and 1,000 Da represents an unknown contamination present in the GBP1 preparation and is thus unrelated to any digested carbohydrate. Digestion assays were performed twice with similar results. UT, untreated; XXXG, xyloglucan heptasaccharide.
 
 ### Figure 3 C) source
-https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065
+https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065
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+
+<img src=dataset\FigS4C.jpg width=60%>
+
+### Supplementary Figure 4 C) caption
+
+**Supplementary Figure 4 C).** Analysis of GBP1-digested β-glucan decasaccharide, extracellular polysaccharides,
+or cell wall fractions from Serendipita indica by MALDI-TOF mass spectrometry. While GBP1 does not act on the EPS matrix and the derived β-GD from S. indica, it releases
+minor oligosaccharide fractions [(m/z 1175 (Hexose7) – m/z 1247 (Hexose13)] from the CW. Oligosaccharide peaks of interest were labeled with m/z (M+Na)+ masses. The digestion assays using the β-GD were performed two times with similar results and the digestion assays using the EPS and CW were performed once. β-GD, β-glucan decasaccharide; CW, cell wall; EPS, extracellular polysaccharides.
+
+### Supplementary Figure 4 C) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
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assays/OxidativeBurstAssay/README.md

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 Apoplastic ROS accumulation after treatment of N. benthamiana (A) and barley (B) with gradually digested laminarin (1 mg·mL−1) was monitored using a luminol-based chemiluminescence assay. Milli-Q water (mock) treatment, untreated laminarin (UT), and GBP1E500A were used as controls. Values represent mean from eight replicates. Boxplots display total ROS accumulation over the measured period of time. The assays were performed two times with independent laminarin digestions. Boxplot elements in this figure: center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. Different letters represent statistically significant differences in relative luminescence units (RLU) based on a one-way ANOVA and Tukey’s post hoc test (significance threshold: p ≤ 0.05). ROS, reactive oxygen species; RLU, relative luminescence units.
 
 ### Figure 5 source
-https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1
+https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1
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+
+<img src=dataset\FigS1A.jpg width=60%>
+
+### Supplementary Figure 1 A) caption
+
+**Supplementary Figure 1 A).**  Barley leaf and root tissues respond similarly to laminarin treatment. Apoplastic ROS accumulation after treatment of seven-day-old barley root pieces and leaf discs with chitohexaose (10 µM) and laminarin (4 mg·mL−1). Treatment
+with Milli-Q water was used as mock control. Values represent mean ± SEM from 16 wells. The experiment was repeated at least three times with similar results.
+
+### Supplementary Figure 1 A) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS1B.jpg width=60%>
+
+### Supplementary Figure 1 B) caption
+
+**Supplementary Figure 1 B).**  Apoplastic ROS accumulation after treatment of two-week-old barley leaf discs with
+untreated and biotinylated laminarin (each 6 mg·mL−1). Treatment with Milli-Q water was used as mock control. Values represent mean ± SEM from 8 wells.
+
+### Supplementary Figure 1 B) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS5B.jpg width=60%>
+
+### Supplementary Figure 5 B) caption
+
+**Supplementary Figure 5 B).** Apoplastic
+ROS accumulation after treatment of barley root pieces with laminarin from either Laminaria digitata (low frequency of β-1,6 linked branches) or E. bicyclis (high frequency of β-1,6 linked branches) was monitored by ROS burst assay. Treatment with Milli-Q water (mock) was used as control. Values represent mean ± SEM from eight wells.
+
+### Supplementary Figure 5 B) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS5C.jpg width=60%>
+
+### Supplementary Figure 5 C) caption
+
+**Supplementary Figure 5 C).** ROS burst assays in the barley control and gbp1 gbp2 mutant lines. Apoplastic ROS accumulation after treatment of barley roots with laminariheptaose (250 µM) or laminarin (4 mg·mL−1) was quantified in the control line and gbp1 gbp2 mutant lines. Treatment with Milli-Q water (mock) was used as control. Values represent mean ± SEM from 16 wells. The experiment was performed twice with similar results. ROS, reactive oxygen species; RLU, relative luminescence units; UT, untreated.
+
+### Supplementary Figure 5 C) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
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assays/StainingForConfocalMicroscopy/README.md

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 **Figure 6 A).** Barley root CW responses of control and *gbp1 gbp2* mutant lines colonized by *S. indica*. Samples were fluorescently labeled with concanavalin A (Con A-AF633, cyan) and wheat germ agglutinin (WGA-AF488, green) for visualization of CWAs and fungal structures, respectively, then analyzed by confocal laser scanning microscopy.
 
 ### Figure 6 A) source
-https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1
+https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1
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+
+<img src=dataset\FigS3A.jpg width=60%>
+
+### Supplementary Figure 3 A) caption
+
+**Supplementary Figure 3 A).** Example of *R. irregularis* structures in barley roots at 28 dpi. *R. irregularis* structures were stained with 5% ink (Pelikan) and fungal structures were observed using a light microscope (AxioStar, Carl Zeiss, Jena, Germany) at 10X magnification.
+
+### Supplementary Figure 3 A) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS6A.jpg width=60%>
+
+### Supplementary Figure 6 A) caption
+
+**Supplementary Figure 6 A).** The number
+and size of papillae upon *S. indica* colonization does not differ between the control and *gbp1 gbp2* mutant lines. *S. indica*-colonized roots of the barley control line and
+*gbp1 gbp2* mutant lines were stained with concanavalin A (ConA-AF633, cyan) and wheat germ agglutinin (WGA-AF488, green) for visualization of papillae and fungal structures, respectively. Images were acquired using a confocal microscope. The diameter of papillae found in the control and *gbp1 gbp2* mutant lines colonized by *S. indica* (n = 61, upper boxplot). The number of papillae were quantified in the control line and *gbp1 gbp2* mutant lines colonized by *S. indica* (n = 25, lower boxplot). Different letters represent statistically significant differences based on one-way ANOVA (significance threshold: p ≤0.05).
+
+### Supplementary Figure 6 A) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS6B.jpg width=60%>
+
+### Supplementary Figure 6 B) caption
+
+**Supplementary Figure 6 B).** The barley control and *gbp1 gbp2* mutant lines do not form spontaneous papillae or CW responses in absence of fungal colonization. Mock-treated roots of the control and *gbp1 gbp2* mutant lines were
+stained with concanavalin A (ConA-AF633, cyan) and wheat germ agglutinin (WGAAF488, green) for visualization of CWAs and fungal structures, respectively. Images were acquired using a confocal microscope.
+
+### Supplementary Figure 6 B) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
+
+<img src=dataset\FigS6C.jpg width=60%>
+
+### Supplementary Figure 6 C) caption
+
+**Supplementary Figure 6 C).** Aniline blue stained callose deposition differs in control and *gbp1 gbp2* mutant lines during *S. indica* colonization. Barley control and *gbp1 gbp2* mutant lines were inoculated with sterile water (mock) or *S. indica* and grown under axenic conditions. Root samples were harvested at 6 dpi and stained with aniline blue to visualize callose deposition. Images were acquired with a confocal microscope. ConA, concanavalin A; CW(A), cell wall (appositions); WGA, wheat germ agglutinin.
+
+### Supplementary Figure 6 C) source
+https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
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