**Figure 5.** GBP1 treatment of long-chain β-1,3-linked glucans modulates ROS production
Apoplastic ROS accumulation after treatment of N. benthamiana (A) and barley (B) with gradually digested laminarin (1 mg·mL−1) was monitored using a luminol-based chemiluminescence assay. Milli-Q water (mock) treatment, untreated laminarin (UT), and GBP1E500A were used as controls. Values represent mean from eight replicates. Boxplots display total ROS accumulation over the measured period of time. The assays were performed two times with independent laminarin digestions. Boxplot elements in this figure: center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. Different letters represent statistically significant differences in relative luminescence units (RLU) based on a one-way ANOVA and Tukey’s post hoc test (significance threshold: p ≤ 0.05). ROS, reactive oxygen species; RLU, relative luminescence units.
