added figures and protocols

assays/BarleyPhenotyping/README.md

+## Figure S2. 
+
+Quantification of root and shoot tissue of the barley control and gbp1 gbp2 mutant lines. Related to Figure 1. (A) The control line and gbp1 gbp2 mutant lines were germinated on wet filter paper for 4 days and then grown on 1/10 PNM medium for 6 days under sterile conditions. Root and shoot fresh weight of the 10-day-old barley plants were measured. Black dots represent biological replicates, and the red bar indicates the average fresh weight (n = 4, each replicate consists of 4 barley plants). Different letters represent statistically significant differences based on one-way ANOVA (significance threshold: p ≤0.05). (B) Representative images of barley control and gbp mutant lines grown on 1/10 PNM under sterile conditions for 7 days. In total, 12 seedlings were grown for each genotype (scale bar = 3 cm). (C) The control line and gbp1 gbp2 lines were germinated on wet filter paper for 4 days and then grown in pre-sterilized soil in pots for 28 days. Root and shoot fresh weight as well as length of the 32-day-old barley plants were measured. Black dots represent biological replicates, and the red bar indicates the average fresh weight (left graphs) or length (right graphs) (n = 5). Different letters represent statistically significant differences based on one-way ANOVA (significance threshold: p ≤0.05).
+
+Source: Supplemental information can be found online at https://doi.org/10.1016/j.cub.2023.10.048
\ No newline at end of file

assays/BarleyPhenotyping/protocols/BarleyMaterialAndGrowthConditions.md

+## Barley plant material 
+
+All experiments, including the generation of CRISPR/Cas9 knock-out lines, were performed with the spring barley (H. vulgare L.) cv. Golden Promise Fast, an introgression line carrying the Ppd-H1 allele that confers fast flowering. 42 From here on, we use ‘‘control’’ to name this non-mutagenized cultivar that carries normal copies of GBP1 and GBP2. 
+
+## Barley growth conditions 
+
+Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands). 82 Seedlings were cultured for four days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 C/18 C, light intensity of 108 mmol$m2$s1 ).
\ No newline at end of file