add mono-associations assay and protocol

assays/Mono-associationsOfSynComMembersWithHostPlants/protocols/Mono-associationsOfSynComMembersWithHostPlantsProtocol.md

+## Mono-associations of SynCom members with host plants
+
+*Lotus* seeds were sterilized and placed on sterile wet Whatman paper for germination. Seedlings were transferred to squared petri dishes containing 0.25× B&D medium (with Fe-EDTA instead of Fe-citrate) supplemented with 3 mM KNO3 and 1% Difco Bacteriological agar, and sterile filter paper was put on top of the sloped solidified medium before placing the seedlings to prevent root growth inside the agar. *Arabidopsis* seeds were sterilized and germinated on 0.5× MS medium plus 1% Difco Bacteriological agar. Seedlings were transferred to squared petri dishes containing 0.5× MS medium (neutral pH, buffered with 2 mM HEPES) plus 1% agar. The 32 strains of the mixed community *LjAt*-SC3 were grown individually in liquid medium, harvested and adjusted to an OD600 of 0.02. Seedlings were inoculated by adding 500 µl of bacterial culture to the roots. Plants were grown for 14 d under long-day conditions (16/8 day–night cycles) at 21 °C. Three biological replicates were prepared for each genotype–bacteria combination.
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