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plant_material_growth_conditions.md 1.67 KiB

Plant material and growth conditions

plant material

species: Hordeum vulgare

parent genotypes:

  • lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa
  • lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa
  • lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa
  • GP-fast: Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021)
  • BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
  • BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
  • BW(Ppd-H1, eam7): cross of the two introgression lines

growth condtions

parents:

  • long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)

  • 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3)

  • Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)

  • F1 crosses:

  • short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)

  • 7x7x7.5 cm pots (pot volume: 367.5 cm^3)

  • Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)

References

Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110. 166249

Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261