Complementation crosses were performed to confirm that *lwd1* and *eam7* are allelic, which are both recessive mutations. For this, homozygous *lwd1* mutants *lwd1-26*, *lwd1-390*, and *lwd1-402* were crossed with BW(Ppd-H1, eam7). F1 plants were grown under SD conditions and flowering time was scored. If *lwd1* and *eam7* are allelic, we expected the F1 plants to display the mutant phenotype and be early flowering under SD.
Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.
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Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261
Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261