leaf_sampling.md

Leaf sampling for RNA extraction

• place two 3 mm metal beads into 2.0 ml tube
• always sample the youngest, fully elongated leaf (auricles are clearly visible)
• sample three biological replicates for each genotype and time point
• for each biological replicate: pool the leaf from two plants into one tube
• freeze samples directly in liquid nitrogen
• Store samples at -80°C until needed