added growth conditions and readme

studies/gene_expression_eam7_plants_development/README.md

+**Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.**
+
+Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE. 
+
+Gene expression of FT1, FT2, FT3 and VRN1 were tested.
+
+
+**study overview**
+
+input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD
+
+	-> assay: leaf_sampling
+		output: frozen leaf tissue (3 biol. replicates)
+		
+		-> assay: RNA_extraction
+			output: RNA samples 
+			
+			-> assay: cDNA_synthesis
+				output: cDNA samples
+				
+				-> assay: qRT-PCR
+					output: gene expression data (2 techn. replicates)
+
+
+
+Results can be found in Supplemental Figure S3A-D.
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studies/gene_expression_eam7_plants_development/protocols/development_plant_material_growth_conditions.md

+**eam7 developmental expression: plant material and growth conditions**
+
+**plant material*
+
+species: Hordeum vulgare
+genotypes:
+
+- BW:  Bowman, spring cultivar
+- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(Ppd-H1, eam7): cross of the two introgression lines
+
+
+**growth condtions**
+
+- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+**sampling**
+
+- always sample youngest, fully elongated leaf
+- sample at ZT 9 (1h after lights turned off, as FT1 expression is highest)
+- sample genotypes at 19, 26, 33, 40, 47 and 59 DAE
+
+
+**References**
+
+Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
+
+Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261
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