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CEPLAS
Helmsorig-2024-eam7
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1e8a0e3c
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1e8a0e3c
authored
5 months ago
by
Gesa Helmsorig
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studies/gene_expression_lwd1_mutants_diurnal/README.md
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@@ -2,24 +2,18 @@
2-week old plants from the genotypes
*lwd1-26*
,
*lwd1-390*
,
*lwd1-402*
, and GP-fast were sampled at Zeitgeber Time (ZT) 0, 4, 8, 12, 16, and 20.
Gene expression of CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1, and PRR1 were tested.
Gene expression of
*
CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1
*
, and
*
PRR1
*
were tested.
**Study overview:**
input: 2-weeks old lwd1-26, lwd1-390, lwd1-402 and GP-fast plants grown under SD.
-> assay: leaf_sampling
output: frozen leaf tissue (3 biol. replicates)
-> assay: RNA_extraction
output: RNA samples
-> assay: cDNA_synthesis
output: cDNA samples
-> assay: qRT-PCR
output: gene expression data (2 techn. replicates)
```
mermaid
flowchart TD;
A[lwd1 mutants and GP-fast] --> |leaf_sampling| B[frozen leaf tissues, 3 biol. replicates]
B --> |RNA_extraction| C[RNA samples]
C --> |cDNA_synthesis| D[cDNA samples]
D --> |qRT-PCR| E[gene expression data, 2 techn. replicates]
```
**Publication:**
Results can be found in Figure 5D-L.
\ No newline at end of file
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