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Commit 1e8a0e3c authored by Gesa Helmsorig's avatar Gesa Helmsorig
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Update README.md

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2-week old plants from the genotypes *lwd1-26*, *lwd1-390*, *lwd1-402*, and GP-fast were sampled at Zeitgeber Time (ZT) 0, 4, 8, 12, 16, and 20.
Gene expression of CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1, and PRR1 were tested.
Gene expression of *CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1*, and *PRR1* were tested.
**Study overview:**
input: 2-weeks old lwd1-26, lwd1-390, lwd1-402 and GP-fast plants grown under SD.
-> assay: leaf_sampling
output: frozen leaf tissue (3 biol. replicates)
-> assay: RNA_extraction
output: RNA samples
-> assay: cDNA_synthesis
output: cDNA samples
-> assay: qRT-PCR
output: gene expression data (2 techn. replicates)
```mermaid
flowchart TD;
A[lwd1 mutants and GP-fast] --> |leaf_sampling| B[frozen leaf tissues, 3 biol. replicates]
B --> |RNA_extraction| C[RNA samples]
C --> |cDNA_synthesis| D[cDNA samples]
D --> |qRT-PCR| E[gene expression data, 2 techn. replicates]
```
**Publication:**
Results can be found in Figure 5D-L.
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