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Commit 32cfeca0 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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added protocol

parent d5d1c7fa
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**crossing of plants**
- grow parent plants in LD conditions
- if parental plants have very different flowering times: sow the faster one with a bit of delay)
- emasculate florets from mother plants before pollen are shed (about Waddington stage 8.5 - 9.0)
- pollinate florets with pollen from father plants, pollinate all florets from one spike with the same father: this is one cross
- cover the spike with a paper bag, count later how many of the pollinated florets develop into seeds
- harvest seeds from each spike / each cross individually when they are completely dry
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