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Commit 3380b70d authored by Gesa Helmsorig's avatar Gesa Helmsorig
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- /studies/phenotyping_lwd1_mutants/protocols/plant_material_growth_conditions.md
- /studies/phenotyping_lwd1_mutants/README.md
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**Phenotype *lwd1* mutants under SD conditions** ## Phenotype *lwd1* mutants under SD conditions
Grow *lwd* mutants and the wild-type GP-fast under short-day (SD) conditions. Score flowering time and phenotypic traits at flowering and at harvest when plants are fully dry. Grow *lwd* mutants and the wild-type GP-fast under short-day (SD) conditions. Score flowering time and phenotypic traits at flowering and at harvest when plants are fully dry.
**study overview** **Study overview:**
input: lwd1-26, lwd1-390, lwd1-402, GP-fast (WT) input: lwd1-26, lwd1-390, lwd1-402, GP-fast (WT)
-> assay: plant_phenotyping -> assay: plant_phenotyping
output: output:
- flowering time (in DAE) - flowering time (in DAE)
- at flowering: plant height, leaf number, tiller number - at flowering: plant height, leaf number, tiller number
- at harvest (on main culm): spike length, floret and grain number, spike fertility - at harvest (on main culm): spike length, floret and grain number,
spike fertility
Results can be found in Figure 5A-C and Supplemental Figure S7. Results can be found in Figure 5A-C and Supplemental Figure S7.
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**Plant material and growth conditions** ## Plant material and growth conditions
**plant material* **Plant material:**
species: Hordeum vulgare Species: *Hordeum vulgare*
genotypes:
Genotypes:
- GP-fast: WT, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) - GP-fast: WT, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021)
- lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa - lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa
- lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa - lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa
- lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa - lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa
**growth condtions** **Growth condtions:**
- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) - QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
**References** **References:**
Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261 Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261
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