- DNA was extracted from leaves of all mutants using QIAGEN_DNeasy-Plant_mini protocol
- plants were tested for possible mutations in the CDS of LWD1 by amplifying the full genomic sequence using the primers LWD1_71us_F (5'-ATCAGACCCCTCCAACGACT-3') and LWD1_21ds_R (5'-TTCCTCTTTCTACCCCACCA-3')
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@@ -7,5 +9,5 @@
- plants transformed with pMGE599/182-1222 (approach 2): LWD1_qPCR114F (5'-CAGCTCATAGCCCATGACAA-3')
- mutations and the zygosity of the mutations were determined relative to the start codon of LWD1
**only in T1 plants**:
**Only in T1 plants:**:
- PCR was performed on transformation vector to check presence/absence of Cas9 using the primers pMGE599_M13F (5'-GTTTTCCCAGTCACGAC-3') and pMGE599_R (5'-TGTCGACATCGATAAGCTTGAA-3')
