added assay and data

3a09bdac · Gesa Helmsorig · 70c9d318 · 3a09bdac · 3a09bdac · 3a09bdac
Commit 3a09bdac authored 8 months ago by Gesa Helmsorig
--- a/assays/RNA_extraction/dataset/eam7_diurnal_RNA_concentration.xlsx
+++ b/assays/RNA_extraction/dataset/eam7_diurnal_RNA_concentration.xlsx
--- a/assays/RNA_extraction/isa.assay.xlsx
+++ b/assays/RNA_extraction/isa.assay.xlsx
--- a/assays/cDNA_synthesis/dataset/eam7_diurnal_cDNA_volumes.xlsx
+++ b/assays/cDNA_synthesis/dataset/eam7_diurnal_cDNA_volumes.xlsx
--- a/assays/cDNA_synthesis/isa.assay.xlsx
+++ b/assays/cDNA_synthesis/isa.assay.xlsx
--- a/assays/leaf_sampling/README.md
+++ b/assays/leaf_sampling/README.md
--- a/assays/leaf_sampling/dataset/.gitkeep
+++ b/assays/leaf_sampling/dataset/.gitkeep
--- a/assays/leaf_sampling/isa.assay.xlsx
+++ b/assays/leaf_sampling/isa.assay.xlsx
--- a/assays/leaf_sampling/protocols/.gitkeep
+++ b/assays/leaf_sampling/protocols/.gitkeep
--- a/assays/leaf_sampling/protocols/leaf_sampling.txt
+++ b/assays/leaf_sampling/protocols/leaf_sampling.txt
+leaf sampling for RNA extraction
+
+-	place two 3 mm metal beads into 2.0 ml tube
+-	always sample the youngest, fully elongated leaf (auricles are clearly visible)
+-	sample three biological replicates for each genotype and time point
+-	for each biological replicate: pool the leaf from two plants into one tube
+-	freeze samples directly in liquid nitrogen
+-	Store samples at -80°C until needed
\ No newline at end of file
--- a/studies/phenotyping_eam7_plants/isa.study.xlsx
+++ b/studies/phenotyping_eam7_plants/isa.study.xlsx