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Commit 4f05797b authored by Gesa Helmsorig's avatar Gesa Helmsorig
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Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence.
**study overview** **study overview**
WT transformation plants (GP-fast) WT transformation plants (GP-fast)
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*study overview* Using CRISPR-Cas9 to generate *lwd1* mutants to confirm LWD1 as the gene underlying the *eam7* locus. Mutants were genotyped by Sanger sequencing.
**study overview**
T0 mutant plants (from study "generating_lwd1_mutants" T0 mutant plants (from study "generating_lwd1_mutants"
-> assay: mutant_genotyping
output: T0 mutant genotype info -> assay: mutant_genotyping
-> assay: plant propagation output: T0 mutant genotype info
output: T1 mutant seeds
-> assay: mutant_genotyping -> assay: plant propagation
output: T1 mutant genotype info output: T1 mutant seeds
-> assay: plant_genotyping
output: T1 mutant genotype info
-> assay: plant propagation -> assay: plant propagation
output: T2 mutant seeds (used in studies "gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants) output: T2 mutant seeds (used in studies gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants)
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