changed protocol

assays/biparental_mapping/protocols/CAPS_marker_analysis.txt

@@ -8,21 +8,21 @@ digest:
 - digesting 5µl of PCR fragments with the CAPS-marker specific restriction enzyme:
 
 
-Marker		Primer	Sequence 5’-3’				Enzyme		annealing temp (°C)		EAM7 allele: fragment size (bp)			eam7 allele: fragment size (bp)
-M-13		F		GTAATTGCCCTAGCGTTGGA	MnlI			58°C					246, 98								328	
+Marker		Primer	Sequence 5’-3’			Enzyme		annealing temp (°C)		EAM7 allele: fragment size (bp)		eam7 allele: fragment size (bp)
+M-13		F		GTAATTGCCCTAGCGTTGGA	MnlI		58°C					246, 98								328	
 			R		CAGAACCCGGGAATTAGGAT											
 M-16		F		AAAACGGTGGCATTAGCAAG	HindIII		58°C					378									214, 168	
 			R		CGGAGGCATGATCAGGTATT																	
-M-11		F		AAAGGCCCACACTAGTGCAT	BstUI		58°C					209, 128								337	
+M-11		F		AAAGGCCCACACTAGTGCAT	BstUI		58°C					209, 128							337	
 			R		ACGCTCACTCCCTGCATCT												
 M-39		F		CTAAATTTCAGCCCCGAACA	DdeI		55°C					237,100								334	
 			R		TGCCTGGACTTGTTCTGATG											
-M-PRR1		F		GAGCATAGCATGGCACTTCA	Hpy188I		58°C					224, 158								383	
+M-PRR1		F		GAGCATAGCATGGCACTTCA	Hpy188I		58°C					224, 158							383	
 			R		GCCTGCCTAACAAACTGACC											
-lwd1-del		F		TACCATCCTCCCATTGCCTG	-			60°C	 				199									167	
+lwd1-del	F		TACCATCCTCCCATTGCCTG	-			60°C	 				199									167	
 			R		ATTGGCTTACACTGCAGGGG						
 
 
-Marker		Primer	Sequence 5’-3’			Enzyme			annealing temp (°C)		ppd-H1 allele: fragment size (bp)			Ppd-H1 allele: fragment size (bp)
-M-PPDH11	F		ACGTGAATGGTGGATCGGC		BstUI	60°C					506									433, 73
+Marker		Primer	Sequence 5’-3’			Enzyme		annealing temp (°C)		ppd-H1 allele: fragment size (bp)	Ppd-H1 allele: fragment size (bp)
+M-PPDH11	F		ACGTGAATGGTGGATCGGC		BstUI		60°C					506									433, 73
 			R		TATAGCTAGGTGCGTGGCG