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edited growth conditions / readme

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studies/allelism_test/protocols/plant_material_growth_conditions.md
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**Plant material and growth conditions** ## Plant material and growth conditions
**plant material** **Plant material:**
species: Hordeum vulgare Species: Hordeum vulgare
parent genotypes: Parent genotypes:
- lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa - *lwd1-26*: lwd1 mutant in GP-fast background, truncated protein of 26 aa
- lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa - *lwd1-390*: lwd1 mutant in GP-fast background, truncated protein of 390 aa
- lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa - *lwd1-402*: lwd1 mutant in GP-fast background, truncated protein of 402 aa
- GP-fast: Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) - GP-fast: Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021)
- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) - BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
- BW(Ppd-H1, eam7): cross of the two introgression lines - BW(Ppd-H1, eam7): cross of the two introgression lines
**growth condtions** **Growth condtions:**
parents: Parents:
- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3) - 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
- F1 crosses: F1 crosses:
- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- 7x7x7.5 cm pots (pot volume: 367.5 cm^3) - 7x7x7.5 cm pots (pot volume: 367.5 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
**References** **References:**
Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110. Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.
166249 166249
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studies/gene_expression_eam7_plants_development/README.md
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**Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.** ## Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.
Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE. Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE.
Gene expression of FT1, FT2, FT3 and VRN1 were tested. Gene expression of FT1, FT2, FT3 and VRN1 were tested.
**study overview** **Study overview:**
input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD
-> assay: leaf_sampling -> assay: leaf_sampling
output: frozen leaf tissue (3 biol. replicates) output: frozen leaf tissue (3 biol. replicates)
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studies/gene_expression_eam7_plants_development/protocols/development_plant_material_growth_conditions.md
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**eam7 developmental expression: plant material and growth conditions** ## *eam7* developmental expression: plant material and growth conditions
**plant material* **Plant material**
species: Hordeum vulgare Species: Hordeum vulgare
genotypes:
Genotypes:
- BW: Bowman, spring cultivar - BW: Bowman, spring cultivar
- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
...@@ -11,20 +12,20 @@ genotypes: ...@@ -11,20 +12,20 @@ genotypes:
- BW(Ppd-H1, eam7): cross of the two introgression lines - BW(Ppd-H1, eam7): cross of the two introgression lines
**growth condtions** **Growth condtions:**
- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3) - QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
**sampling** **Sampling:**
- always sample youngest, fully elongated leaf - always sample youngest, fully elongated leaf
- sample at ZT 9 (1h after lights turned off, as FT1 expression is highest) - sample at ZT 9 (1h after lights turned off, as FT1 expression is highest)
- sample genotypes at 19, 26, 33, 40, 47 and 59 DAE - sample genotypes at 19, 26, 33, 40, 47 and 59 DAE
**References** **References:**
Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249 Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
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