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Commit 72a61c9f authored by Gesa Helmsorig's avatar Gesa Helmsorig
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## Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development. ## Test gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.
Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE. Genotypes BW, BW(*Ppd-H1*, BW(*eam7*), and BW(*Ppd-H1,eam7*) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE.
Gene expression of FT1, FT2, FT3 and VRN1 were tested.
Gene expression of *FT1*, *FT*2, *FT3* and *VRN1* were tested.
**Study overview:** **Study overview:**
input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD ```mermaid
flowchart TD;
-> assay: leaf_sampling A[Bowman and introgression lines] --> |leaf_sampling| B[frozen leaf tissues, 3 biol. replicates]
output: frozen leaf tissue (3 biol. replicates) B --> |RNA_extraction| C[RNA samples]
C --> |cDNA_synthesis| D[cDNA samples]
-> assay: RNA_extraction D --> |qRT-PCR| E[gene expression data, 2 techn. replicates]
output: RNA samples ```
-> assay: cDNA_synthesis
output: cDNA samples
-> assay: qRT-PCR
output: gene expression data (2 techn. replicates)
**Publication:**
Results can be found in Supplemental Figure S3A-D. Results can be found in Supplemental Figure S3A-D.
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