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Commit 986d4755 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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added metatadata + data for MSA dissection SD/LD

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- per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD)
- start 5 days after emergence (DAE)
- remove the leaves surrounding the main shoot apex (MSA) with a blade
- image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera
- score developmental stage according to Waddington et al. (1983)
- count spikelet meristems (include those that have developet into floret meristems or florets) on the MSA
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Scoring of the development according to:
Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434
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**Plant material and growth conditions** **Plant material and growth conditions**
**plant material** **plant material**
species: Hordeum vulgare species: Hordeum vulgare
genotypes: genotypes:
- BW: Bowman, spring cultivar - BW: Bowman, spring cultivar
- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) - BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
- BW(Ppd-H1, eam7): cross of the two introgression lines - BW(Ppd-H1, eam7): cross of the two introgression lines
**growth condtions** **growth condtions**
- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) - QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
**plants** **plants**
- SD: sample names include "SD" - SD: plant 1 - 204
- LD: sample names include "LD" - LD: plant 205 - 342
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