added plant material and growth conditions

studies/biparental_mapping_eam7/protocols/plant_material_growth_conditions.md

+**Plant material and growth conditions**
+
+**plant material**
+
+species: Hordeum vulgare
+
+genotypes: 
+- BW: Bowman, spring cultivar
+- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(Ppd-H1, eam7): cross of the two introgression lines
+
+
+**growth condtions**
+
+- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+
+**References**
+
+Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.
+166249

studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md

@@ -21,7 +21,7 @@ genotypes:
 **sampling**
 
 - 2 weeks old plants
-- youngest, fully elongated leaf: leaf 
+- youngest, fully elongated leaf: leaf 2
 - sample every 2 hours over 24 hours: 
 	ZT0 (lights are turned on)
 	ZT2

studies/gene_expression_lwd1_mutants_diurnal/protocols/diurnal_plant_material_growth_conditions.md

+**eam7 diurnal expression: plant material and growth conditions**
+
+**plant material*
+
+species: Hordeum vulgare
+genotypes:
+
+- GP-fast: WT, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) 
+- lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa
+- lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa
+- lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa
+
+
+
+**growth condtions**
+
+- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+
+**sampling**
+
+- 2 weeks old plants
+- youngest, fully elongated leaf: leaf 2
+- sample every 2 hours over 24 hours: 
+	ZT0 (lights are turned on)
+	ZT4
+	ZT8 (lights are turned off)
+	ZT12
+	ZT16
+	ZT20
\ No newline at end of file

studies/generating_lwd1_mutants/protocols/plant_material_growth_conditions.md

+**Plant material and growth conditions**
+
+**plant material**
+
+species: Hordeum vulgare
+
+
+- GP-fast: Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) 
+
+**growth condtions**
+
+parents: 
+- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+- F1 crosses: 
+- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- 7x7x7.5 cm pots (pot volume:  367.5 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+
+**References**
+
+Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.
+166249
+
+Gol L, Haraldsson EB, von Korff M. Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley. J Exp Bot. 2021:72(1):122–136. https://doi.org/10.1093/jxb/eraa261