re-structured metadata

b4891b5e · Gesa Helmsorig · 18d70cf8 · b4891b5e · b4891b5e · b4891b5e
Commit b4891b5e authored 5 months ago by Gesa Helmsorig
--- a/assays/embryo_transformation/dataset/transformation_T0_results.xlsx
+++ b/assays/embryo_transformation/dataset/transformation_T0_results.xlsx
--- a/assays/embryo_transformation/isa.assay.xlsx
+++ b/assays/embryo_transformation/isa.assay.xlsx
--- a/assays/embryo_transformation/protocols/cloning_trafo_protocol.md
+++ b/assays/embryo_transformation/protocols/cloning_trafo_protocol.md
@@ -15,9 +15,6 @@

 - GP-fast plants were cultivated in long-day conditions (16h day / 8 night) in control temperature (20°C / 16°C)
 - immature embryos were used for transformation according to Hensel et al. (2009)
- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol
- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3')
- 


 **References:**

--- a/assays/embryo_transformation/protocols/test_hygromycin.md
+++ b/assays/embryo_transformation/protocols/test_hygromycin.md
+## Test regenerated plants for successful transformation
+
+- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol
+- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3')
+ 
--- a/studies/generating_lwd1_mutants/isa.study.xlsx
+++ b/studies/generating_lwd1_mutants/isa.study.xlsx