Merge branch helmsorig-2024-eam7:main into main

assays/embryo_transformation/protocols/cloning_trafo_protocol.md

@@ -15,9 +15,6 @@
 
 - GP-fast plants were cultivated in long-day conditions (16h day / 8 night) in control temperature (20°C / 16°C)
 - immature embryos were used for transformation according to Hensel et al. (2009)
-- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol
-- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3')
- 
 
 
 **References:**

assays/embryo_transformation/protocols/test_hygromycin.md

+## Test regenerated plants for successful transformation
+
+- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol
+- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3')
+ 

assays/leaf_sampling/README.md

-## leaf_sampling
-
-**Assay used in studies:**
-- gene_expression_eam7_plants_development
-- gene_expression_eam7_plants_diurnal
-- gene_expression_lwd1_mutants_diurnal
-
-**Info:**
-
-No dataset, as leaf samples were used in downstream assays (RNA_extraction).
\ No newline at end of file

assays/leaf_sampling/protocols/leaf_sampling.md

-## Leaf sampling for RNA extraction
-
--	place two 3 mm metal beads into 2.0 ml tube
--	always sample the youngest, fully elongated leaf (auricles are clearly visible)
--	sample three biological replicates for each genotype and time point
--	for each biological replicate: pool the leaf from two plants into one tube
--	freeze samples directly in liquid nitrogen
--	Store samples at -80°C until needed
\ No newline at end of file