Update 2 files

- /assays/qRT-PCR/protocols/qRT-PCR_primer.txt
- /assays/qRT-PCR/protocols/qRT-PCR_primer.md

assays/qRT-PCR/protocols/qRT-PCR_primer.md

+
+
+| Target gene | Primer | Sequence 5’-3’ | Fragment size (bp) |
+| - | - | - | - |
+| ACT (HORVU.MOREX.r3.5HG0457850) | ACT_591F | CGTGTTGGATTCTGGTGATG | 208| 
+| | ACT_789R | AGCCACATATGCGAGCTTCT | | 
+| CO1 (HORVU.MOREX.r3.7HG0671540) | CO1_2185F | CTGCTGGGGCTAGTGCTTAC | 251| 
+| | CO1_3454R | CCTTGTTGCATAACGTGTGG | |
+| CO2 (HORVU.MOREX.r3.6HG0611630) | CO2_564F | AGTGGACTCTTGGCTCCTCA | 158 | 
+| | CO2_721R | CATGCTGCTGTTCTTGCATT | |
+| ELF3 (HORVU.MOREX.r3.1HG0095050) | ELF3_DL_3060_F3 | TGCTGTCCAAGTGTTTGAGC | 242 | 
+ | | Elf3_DL_4483_R3 | CCTGGTTTCCTTCGGTGTTA | |
+| FT1 (HORVU.MOREX.r3.7HG0653910) | FT1_1955F | GGTAGACCCAGATGCTCCAA | 120 | 
+| | FT1_2183R | TCGTAGCACATCACCTCCTG | |
+| FT2 (HORVU.MOREX.r3.3HG0244930  | FT2_4031R | TCACATCCTTCTCCCGCCGG | 238 | 
+| | FT2_3809_F | TACCGAGGTTGTGTGCTACG | |
+| FT3 (HORVU.MOREX.r3.1HG0077250) | FT3-1004F | TGTCAGACATCCCTGGAACA | 225 | 
+| | FT3-1848R | CTGATCCACCTTCCCTTTGA | |
+| FT4 (HORVU.MOREX.r3.2HG0117260) | FT4_626F | AAACGATAGCTTTGGAAAAGAGATC | 230 | 
+| | FT4_2537R | TCCTCCCGCCGGTGCCCGTG | |
+| GI (HORVU.MOREX.r3.3HG0238250)  | GI_6780F | TCAGTTAGAGCTCCTGGAAGT | 263 | 
+| | GI_7289R | GGTAGTTTGGGCTTTGGATG | |
+| LHY (HORVU.MOREX.r3.7HG0699010) | HvLHY_672F | CCTGGAATTGGAGATGGAGA | 210 | 
+| | HvLHY_882R | TGAGCATGGCTTCTGATTTG | |
+| LUX1 (HORVU.MOREX.r3.3HG0328340) | LUX_1077F | AATTCAGTCCACGGATGCTC | 222 | 
+| | LUX_1298R | CTTCACTTCAGCTCCCCTTG | |
+| LWD1 (HORVU.MOREX.r3.6HG0583670) | LWD1_qPCR-114_F | CAGCTCATAGCCCATGACAA | 114 | 
+| | LWD1_qPCR-114_R | CTCCTTGTCCCGAAGATCAA | |
+| PHYB (HORVU.MOREX.r3.4HG0381880) | HvPHYB-1151F | CTTGCGCACCAACTATCAGA | 232 | 
+| | HvPHYB-1383R | CTCCATGACACACCGTCAAC | |
+| PHYC (HORVU.MOREX.r3.5HG0511090) | PHYC_0986F | ACTACCCGGCAACTGACATC | 142 | 
+| | PHYC_1127R | GAGCCACAGAGGCTGATAGG | |
+| Ppd-H1 (HORVU.MOREX.r3.2HG0107710) | PPD1_2165F | GATGGATTCAAAGGCAAGGA | 172 | 
+| | PPD1_2336R | GAACAATTGGCTCCTCCAAA | |
+| PRR1 (HORVU.MOREX.r3.6HG0595250) | HvPRR1_1056F | GAGCATAGCATGGCACTTCA | 237 | 
+| | HvPRR1_1292R | TGTCTTTCCTCGGAAATTGG | | 
+| PRR59 (HORVU.MOREX.r3.4HG0350690) | PRR59_2064_F | GAAATTCCGCATGAAAAGGA | 148 | 
+| | PRR59_2212_R | TTCCGCATCTTCTGTTGTTG | |
+| PRR73 (HORVU.MOREX.r3.5HG0498830) | PRR73_441F | GCGCCGTAGAGAATCAGAAC | 222 | 
+| | PRR73_662R | CATGTCGGGTACAGTCATCG | |
+| PRR95 (HORVU.MOREX.r3.5HG0498830) | PRR95_1467F | CAGAACTCCAGTGTCGCAAA | 251 | 
+| | PRR95_1717R | TGCTGTTGCCAGAGTTGTTC | |
+| VRN1 (HORVU.MOREX.r3.5HG0511210) | HVBM5a_292F | CTGAAGGCGAAGGTTGAGAC | 203 | 
+| | HVBM5a_494R | TTCTCCTCCTGCAGTGACCT | |
\ No newline at end of file

assays/qRT-PCR/protocols/qRT-PCR_primer.txt

-Target gene							Primer				Sequence 5’-3’					Fragment size (bp)
-ACT (HORVU.MOREX.r3.5HG0457850)	ACT_591F			CGTGTTGGATTCTGGTGATG		208
-									ACT_789R			AGCCACATATGCGAGCTTCT	
-CO1 (HORVU.MOREX.r3.7HG0671540)	CO1_2185F			CTGCTGGGGCTAGTGCTTAC		251
-									CO1_3454R			CCTTGTTGCATAACGTGTGG	
-CO2 (HORVU.MOREX.r3.6HG0611630)	CO2_564F			AGTGGACTCTTGGCTCCTCA		158
-									CO2_721R			CATGCTGCTGTTCTTGCATT	
-ELF3 (HORVU.MOREX.r3.1HG0095050)	ELF3_DL_3060_F3	TGCTGTCCAAGTGTTTGAGC		242
-									Elf3_DL_4483_R3		CCTGGTTTCCTTCGGTGTTA	
-FT1 (HORVU.MOREX.r3.7HG0653910)	FT1_1955F			GGTAGACCCAGATGCTCCAA		120
-									FT1_2183R			TCGTAGCACATCACCTCCTG	
-FT2 (HORVU.MOREX.r3.3HG0244930		FT2_4031R			TCACATCCTTCTCCCGCCGG		238
-									FT2_3809_F			TACCGAGGTTGTGTGCTACG
-FT3 (HORVU.MOREX.r3.1HG0077250)	FT3-1004F			TGTCAGACATCCCTGGAACA		225
-									FT3-1848R			CTGATCCACCTTCCCTTTGA	
-FT4 (HORVU.MOREX.r3.2HG0117260)	FT4_626F			AAACGATAGCTTTGGAAAAGAGATC	230
-									FT4_2537R			TCCTCCCGCCGGTGCCCGTG
-GI (HORVU.MOREX.r3.3HG0238250)		GI_6780F			TCAGTTAGAGCTCCTGGAAGT		263
-									GI_7289R			GGTAGTTTGGGCTTTGGATG	
-LHY (HORVU.MOREX.r3.7HG0699010)	HvLHY_672F			CCTGGAATTGGAGATGGAGA		210
-									HvLHY_882R			TGAGCATGGCTTCTGATTTG	
-LUX1 (HORVU.MOREX.r3.3HG0328340)	LUX_1077F			AATTCAGTCCACGGATGCTC		222
-									LUX_1298R			CTTCACTTCAGCTCCCCTTG	
-LWD1 (HORVU.MOREX.r3.6HG0583670)	LWD1_qPCR-114_F	CAGCTCATAGCCCATGACAA		114
-									LWD1_qPCR-114_R	CTCCTTGTCCCGAAGATCAA	
-PHYB (HORVU.MOREX.r3.4HG0381880)	HvPHYB-1151F		CTTGCGCACCAACTATCAGA		232
-									HvPHYB-1383R		CTCCATGACACACCGTCAAC	
-PHYC (HORVU.MOREX.r3.5HG0511090)	PHYC_0986F			ACTACCCGGCAACTGACATC		142
-									PHYC_1127R 		GAGCCACAGAGGCTGATAGG		
-Ppd-H1 (HORVU.MOREX.r3.2HG0107710)	PPD1_2165F			GATGGATTCAAAGGCAAGGA		172
-									PPD1_2336R			GAACAATTGGCTCCTCCAAA	
-PRR1 (HORVU.MOREX.r3.6HG0595250)	HvPRR1_1056F		GAGCATAGCATGGCACTTCA		237
-									HvPRR1_1292R		TGTCTTTCCTCGGAAATTGG	
-PRR59 (HORVU.MOREX.r3.4HG0350690)	PRR59_2064_F		GAAATTCCGCATGAAAAGGA		148
-									PRR59_2212_R		TTCCGCATCTTCTGTTGTTG	
-PRR73 (HORVU.MOREX.r3.5HG0498830)	PRR73_441F			GCGCCGTAGAGAATCAGAAC		222
-									PRR73_662R			CATGTCGGGTACAGTCATCG
-PRR95 (HORVU.MOREX.r3.5HG0498830)	PRR95_1467F		CAGAACTCCAGTGTCGCAAA		251
-									PRR95_1717R		TGCTGTTGCCAGAGTTGTTC	
-VRN1 (HORVU.MOREX.r3.5HG0511210)	HVBM5a_292F		CTGAAGGCGAAGGTTGAGAC		203
-									HVBM5a_494R		TTCTCCTCCTGCAGTGACCT	
\ No newline at end of file