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Update file diurnal_plant_material_growth_conditions.md

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**lwd1 mutants diurnal expression: plant material and growth conditions** ## *lwd1* mutants diurnal expression: plant material and growth conditions
**plant material* **Plant material:**
species: Hordeum vulgare Species: *Hordeum vulgare*
genotypes:
- GP-fast: WT, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021) Genotypes:
- GP-fast: wild-type, Ppd-H1 introgression line in Golden Promise background, lwd1 mutant background, Gol et al. (2021)
- lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa - lwd1-26: lwd1 mutant in GP-fast background, truncated protein of 26 aa
- lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa - lwd1-390: lwd1 mutant in GP-fast background, truncated protein of 390 aa
- lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa - lwd1-402: lwd1 mutant in GP-fast background, truncated protein of 402 aa
**growth condtions** **Growth condtions:**
- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3) - QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
**sampling** **Sampling:**
- 2 weeks old plants - 2 weeks old plants
- youngest, fully elongated leaf: leaf 2 - youngest, fully elongated leaf: leaf 2
- sample every 2 hours over 24 hours: - sample every 2 hours over 24 hours:
ZT0 (lights are turned on) - ZT0 (lights are turned on)
ZT4 - ZT4
ZT8 (lights are turned off) - ZT8 (lights are turned off)
ZT12 - ZT12
ZT16 - ZT16
ZT20 - ZT20
**References** **References:**
Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249 Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
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