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Commit f987d946 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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Update CAPS_marker_analysis.md

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assays/biparental_mapping/protocols/CAPS_marker_analysis.md
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...@@ -8,20 +8,23 @@ digest: ...@@ -8,20 +8,23 @@ digest:
- digesting 5µl of PCR fragments with the CAPS-marker specific restriction enzyme: - digesting 5µl of PCR fragments with the CAPS-marker specific restriction enzyme:
Marker Primer Sequence 5’-3’ Enzyme annealing temp (°C) EAM7 allele: fragment size (bp) eam7 allele: fragment size (bp) | Marker | Primer | Sequence 5’-3’ | Enzyme | annealing temp (°C) | EAM7 allele: fragment size (bp) | eam7 allele: fragment size (bp) |
M-13 F GTAATTGCCCTAGCGTTGGA MnlI 58°C 246, 98 328 | ------ | ------ | ------ | ------ | ------ | ------ | ------ |
R CAGAACCCGGGAATTAGGAT | M-13 | F | GTAATTGCCCTAGCGTTGGA | MnlI | 58°C | 246, 98 | 328 |
M-16 F AAAACGGTGGCATTAGCAAG HindIII 58°C 378 214, 168 | | R | CAGAACCCGGGAATTAGGAT |
R CGGAGGCATGATCAGGTATT | M-16 | F | AAAACGGTGGCATTAGCAAG | HindIII | 58°C | 378 | 214, 168 |
M-11 F AAAGGCCCACACTAGTGCAT BstUI 58°C 209, 128 337 | | R | CGGAGGCATGATCAGGTATT |
R ACGCTCACTCCCTGCATCT | M-11 | F | AAAGGCCCACACTAGTGCAT | BstUI | 58°C | 209, 128 | 337|
M-39 F CTAAATTTCAGCCCCGAACA DdeI 55°C 237,100 334 | | R | ACGCTCACTCCCTGCATCT |
R TGCCTGGACTTGTTCTGATG | M-39 | F | CTAAATTTCAGCCCCGAACA | DdeI | 55°C | 237, 100 | 334
M-PRR1 F GAGCATAGCATGGCACTTCA Hpy188I 58°C 224, 158 383 | | R | TGCCTGGACTTGTTCTGATG |
R GCCTGCCTAACAAACTGACC | M-PRR1 | F | GAGCATAGCATGGCACTTCA | Hpy188I | 58°C | 224, 158 | 383
lwd1-del F TACCATCCTCCCATTGCCTG - 60°C 199 167 | | R | GCCTGCCTAACAAACTGACC |
R ATTGGCTTACACTGCAGGGG | lwd1-del | F | TACCATCCTCCCATTGCCTG | - | 60°C | 199 | 167 |
| | R | ATTGGCTTACACTGCAGGGG |
Marker Primer Sequence 5’-3’ Enzyme annealing temp (°C) ppd-H1 allele: fragment size (bp) Ppd-H1 allele: fragment size (bp)
M-PPDH11 F ACGTGAATGGTGGATCGGC BstUI 60°C 506 433, 73 | Marker | Primer | Sequence 5’-3’ | Enzyme | annealing temp (°C) | ppd-H1 allele: fragment size (bp) | Ppd-H1 allele: fragment size (bp) |
R TATAGCTAGGTGCGTGGCG | ------ | ------ | ------ | ------ | ------ | ------ | ------ |
| M-PPDH11 | F | ACGTGAATGGTGGATCGGC | BstUI | 60°C | 506 | 433, 73 |
| | R | TATAGCTAGGTGCGTGGCG |
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