Skip to content
Snippets Groups Projects
Commit 18d70cf8 authored by Gesa Helmsorig's avatar Gesa Helmsorig
Browse files

updated metadata, sample names

parent ee6a1c13
No related branches found
No related tags found
No related merge requests found
Hide whitespace changes
Inline Side-by-side
Showing
with 47 additions and 45 deletions
assays/qRT-PCR/dataset/eam7_development_gene_expression.xlsx
+ 0
− 0
View file @ 18d70cf8
No preview for this file type
assays/qRT-PCR/dataset/eam7_diurnal_gene_expression.xlsx
+ 0
− 0
View file @ 18d70cf8
No preview for this file type
assays/qRT-PCR/dataset/lwd1_diurnal_gene_expression.xlsx
+ 0
− 0
View file @ 18d70cf8
No preview for this file type
assays/qRT-PCR/isa.assay.xlsx
+ 0
− 0
View file @ 18d70cf8
No preview for this file type
assays/qRT-PCR/protocols/qRT-PCR_primer.md deleted 100644 → 0
+ 0
− 44
View file @ ee6a1c13
| Target gene | Primer | Sequence 5’-3’ | Fragment size (bp) |
| - | - | - | - |
| ACT (HORVU.MOREX.r3.5HG0457850) | ACT_591F | CGTGTTGGATTCTGGTGATG | 208|
| | ACT_789R | AGCCACATATGCGAGCTTCT | |
| CO1 (HORVU.MOREX.r3.7HG0671540) | CO1_2185F | CTGCTGGGGCTAGTGCTTAC | 251|
| | CO1_3454R | CCTTGTTGCATAACGTGTGG | |
| CO2 (HORVU.MOREX.r3.6HG0611630) | CO2_564F | AGTGGACTCTTGGCTCCTCA | 158 |
| | CO2_721R | CATGCTGCTGTTCTTGCATT | |
| ELF3 (HORVU.MOREX.r3.1HG0095050) | ELF3_DL_3060_F3 | TGCTGTCCAAGTGTTTGAGC | 242 |
| | Elf3_DL_4483_R3 | CCTGGTTTCCTTCGGTGTTA | |
| FT1 (HORVU.MOREX.r3.7HG0653910) | FT1_1955F | GGTAGACCCAGATGCTCCAA | 120 |
| | FT1_2183R | TCGTAGCACATCACCTCCTG | |
| FT2 (HORVU.MOREX.r3.3HG0244930 | FT2_4031R | TCACATCCTTCTCCCGCCGG | 238 |
| | FT2_3809_F | TACCGAGGTTGTGTGCTACG | |
| FT3 (HORVU.MOREX.r3.1HG0077250) | FT3-1004F | TGTCAGACATCCCTGGAACA | 225 |
| | FT3-1848R | CTGATCCACCTTCCCTTTGA | |
| FT4 (HORVU.MOREX.r3.2HG0117260) | FT4_626F | AAACGATAGCTTTGGAAAAGAGATC | 230 |
| | FT4_2537R | TCCTCCCGCCGGTGCCCGTG | |
| GI (HORVU.MOREX.r3.3HG0238250) | GI_6780F | TCAGTTAGAGCTCCTGGAAGT | 263 |
| | GI_7289R | GGTAGTTTGGGCTTTGGATG | |
| LHY (HORVU.MOREX.r3.7HG0699010) | HvLHY_672F | CCTGGAATTGGAGATGGAGA | 210 |
| | HvLHY_882R | TGAGCATGGCTTCTGATTTG | |
| LUX1 (HORVU.MOREX.r3.3HG0328340) | LUX_1077F | AATTCAGTCCACGGATGCTC | 222 |
| | LUX_1298R | CTTCACTTCAGCTCCCCTTG | |
| LWD1 (HORVU.MOREX.r3.6HG0583670) | LWD1_qPCR-114_F | CAGCTCATAGCCCATGACAA | 114 |
| | LWD1_qPCR-114_R | CTCCTTGTCCCGAAGATCAA | |
| PHYB (HORVU.MOREX.r3.4HG0381880) | HvPHYB-1151F | CTTGCGCACCAACTATCAGA | 232 |
| | HvPHYB-1383R | CTCCATGACACACCGTCAAC | |
| PHYC (HORVU.MOREX.r3.5HG0511090) | PHYC_0986F | ACTACCCGGCAACTGACATC | 142 |
| | PHYC_1127R | GAGCCACAGAGGCTGATAGG | |
| Ppd-H1 (HORVU.MOREX.r3.2HG0107710) | PPD1_2165F | GATGGATTCAAAGGCAAGGA | 172 |
| | PPD1_2336R | GAACAATTGGCTCCTCCAAA | |
| PRR1 (HORVU.MOREX.r3.6HG0595250) | HvPRR1_1056F | GAGCATAGCATGGCACTTCA | 237 |
| | HvPRR1_1292R | TGTCTTTCCTCGGAAATTGG | |
| PRR59 (HORVU.MOREX.r3.4HG0350690) | PRR59_2064_F | GAAATTCCGCATGAAAAGGA | 148 |
| | PRR59_2212_R | TTCCGCATCTTCTGTTGTTG | |
| PRR73 (HORVU.MOREX.r3.5HG0498830) | PRR73_441F | GCGCCGTAGAGAATCAGAAC | 222 |
| | PRR73_662R | CATGTCGGGTACAGTCATCG | |
| PRR95 (HORVU.MOREX.r3.5HG0498830) | PRR95_1467F | CAGAACTCCAGTGTCGCAAA | 251 |
| | PRR95_1717R | TGCTGTTGCCAGAGTTGTTC | |
| VRN1 (HORVU.MOREX.r3.5HG0511210) | HVBM5a_292F | CTGAAGGCGAAGGTTGAGAC | 203 |
| | HVBM5a_494R | TTCTCCTCCTGCAGTGACCT | |
\ No newline at end of file
assays/qRT-PCR/protocols/qRT-PCR_protocol.md
+ 47
− 1
View file @ 18d70cf8
......@@ -55,4 +55,50 @@ If no standard curve was available:
**4. Calculate means and standard deviation for all samples**
- relative expression was averaged over the three biological replicates for each sample
- standard deviation was calculated across the three biological replicates for each sample
\ No newline at end of file
- standard deviation was calculated across the three biological replicates for each sample
**Primer list:**
| Target gene | Primer | Sequence 5’-3’ | Fragment size (bp) |
| - | - | - | - |
| ACT (HORVU.MOREX.r3.5HG0457850) | ACT_591F | CGTGTTGGATTCTGGTGATG | 208|
| | ACT_789R | AGCCACATATGCGAGCTTCT | |
| CO1 (HORVU.MOREX.r3.7HG0671540) | CO1_2185F | CTGCTGGGGCTAGTGCTTAC | 251|
| | CO1_3454R | CCTTGTTGCATAACGTGTGG | |
| CO2 (HORVU.MOREX.r3.6HG0611630) | CO2_564F | AGTGGACTCTTGGCTCCTCA | 158 |
| | CO2_721R | CATGCTGCTGTTCTTGCATT | |
| ELF3 (HORVU.MOREX.r3.1HG0095050) | ELF3_DL_3060_F3 | TGCTGTCCAAGTGTTTGAGC | 242 |
| | Elf3_DL_4483_R3 | CCTGGTTTCCTTCGGTGTTA | |
| FT1 (HORVU.MOREX.r3.7HG0653910) | FT1_1955F | GGTAGACCCAGATGCTCCAA | 120 |
| | FT1_2183R | TCGTAGCACATCACCTCCTG | |
| FT2 (HORVU.MOREX.r3.3HG0244930 | FT2_4031R | TCACATCCTTCTCCCGCCGG | 238 |
| | FT2_3809_F | TACCGAGGTTGTGTGCTACG | |
| FT3 (HORVU.MOREX.r3.1HG0077250) | FT3-1004F | TGTCAGACATCCCTGGAACA | 225 |
| | FT3-1848R | CTGATCCACCTTCCCTTTGA | |
| FT4 (HORVU.MOREX.r3.2HG0117260) | FT4_626F | AAACGATAGCTTTGGAAAAGAGATC | 230 |
| | FT4_2537R | TCCTCCCGCCGGTGCCCGTG | |
| GI (HORVU.MOREX.r3.3HG0238250) | GI_6780F | TCAGTTAGAGCTCCTGGAAGT | 263 |
| | GI_7289R | GGTAGTTTGGGCTTTGGATG | |
| LHY (HORVU.MOREX.r3.7HG0699010) | HvLHY_672F | CCTGGAATTGGAGATGGAGA | 210 |
| | HvLHY_882R | TGAGCATGGCTTCTGATTTG | |
| LUX1 (HORVU.MOREX.r3.3HG0328340) | LUX_1077F | AATTCAGTCCACGGATGCTC | 222 |
| | LUX_1298R | CTTCACTTCAGCTCCCCTTG | |
| LWD1 (HORVU.MOREX.r3.6HG0583670) | LWD1_qPCR-114_F | CAGCTCATAGCCCATGACAA | 114 |
| | LWD1_qPCR-114_R | CTCCTTGTCCCGAAGATCAA | |
| PHYB (HORVU.MOREX.r3.4HG0381880) | HvPHYB-1151F | CTTGCGCACCAACTATCAGA | 232 |
| | HvPHYB-1383R | CTCCATGACACACCGTCAAC | |
| PHYC (HORVU.MOREX.r3.5HG0511090) | PHYC_0986F | ACTACCCGGCAACTGACATC | 142 |
| | PHYC_1127R | GAGCCACAGAGGCTGATAGG | |
| Ppd-H1 (HORVU.MOREX.r3.2HG0107710) | PPD1_2165F | GATGGATTCAAAGGCAAGGA | 172 |
| | PPD1_2336R | GAACAATTGGCTCCTCCAAA | |
| PRR1 (HORVU.MOREX.r3.6HG0595250) | HvPRR1_1056F | GAGCATAGCATGGCACTTCA | 237 |
| | HvPRR1_1292R | TGTCTTTCCTCGGAAATTGG | |
| PRR59 (HORVU.MOREX.r3.4HG0350690) | PRR59_2064_F | GAAATTCCGCATGAAAAGGA | 148 |
| | PRR59_2212_R | TTCCGCATCTTCTGTTGTTG | |
| PRR73 (HORVU.MOREX.r3.5HG0498830) | PRR73_441F | GCGCCGTAGAGAATCAGAAC | 222 |
| | PRR73_662R | CATGTCGGGTACAGTCATCG | |
| PRR95 (HORVU.MOREX.r3.5HG0498830) | PRR95_1467F | CAGAACTCCAGTGTCGCAAA | 251 |
| | PRR95_1717R | TGCTGTTGCCAGAGTTGTTC | |
| VRN1 (HORVU.MOREX.r3.5HG0511210) | HVBM5a_292F | CTGAAGGCGAAGGTTGAGAC | 203 |
| | HVBM5a_494R | TTCTCCTCCTGCAGTGACCT | |
\ No newline at end of file
0% or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment