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Commit 697cc209 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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## Test diurnal gene expression of circadian clock and flowering time genes in Bowman and Bowman-derived introgression lines.
2-week old plants from the genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22.
2-week old plants from the genotypes BW, BW(*Ppd-H1*), BW(*eam7*), and BW(*Ppd-H1,eam7*) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22.
Gene expression of CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95 and VRN1 were tested.
Gene expression of *CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95* and *VRN1* were tested.
**Study overview:**
input: 2-weeks old BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD
-> assay: leaf_sampling
output: frozen leaf tissue (3 biol. replicates)
-> assay: RNA_extraction
output: RNA samples
-> assay: cDNA_synthesis
output: cDNA samples
-> assay: qRT-PCR
output: gene expression data (2 techn. replicates)
```mermaid
flowchart TD;
A[Bowman and introgression lines] --> |leaf_sampling| B[frozen leaf tissues, 3 biol. replicates]
B --> |RNA_extraction| C[RNA samples]
C --> |cDNA_synthesis| D[cDNA samples]
D --> |qRT-PCR| E[gene expression data, 2 techn. replicates]
```
**Publication:**
Results can be found in Figure 3 and in Supplemental Figure S3E.
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