## Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.
## Test gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.
Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE.
Gene expression of FT1, FT2, FT3 and VRN1 were tested.
Genotypes BW, BW(*Ppd-H1*, BW(*eam7*), and BW(*Ppd-H1,eam7*) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE.
Gene expression of *FT1*, *FT*2, *FT3* and *VRN1* were tested.
**Study overview:**
input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD
-> assay: leaf_sampling
output: frozen leaf tissue (3 biol. replicates)
-> assay: RNA_extraction
output: RNA samples
-> assay: cDNA_synthesis
output: cDNA samples
-> assay: qRT-PCR
output: gene expression data (2 techn. replicates)